{"id":"80202290-8b48-5a42-a5e9-6dbc855b0336","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:clemastanin-b-retains-influenza-rnp-in-the-nucleus","predicate":"reduces","statement":"Influenza virus ribonucleoprotein was retained in the nucleus after treatment with clemastanin B, and virus titre fell when cells were treated after infection, particularly at the early stage.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0c74df10-4594-5f73-a6f4-1c81d12b6eae","mechanism_event_label":"Influenza virus ribonucleoprotein was retained in the nucleus after treatment with clemastanin B, and virus titre fell when cells were treated after infection, particularly at the early stage.","subject":{"id":"0526af10-74ff-50d5-9602-125327302c48","slug":"clemastanin-b","display_name":"Clemastanin B","entity_type_key":"small_molecule"},"object":{"id":"425ae2d7-6999-5d2e-97ba-7da5295fb748","slug":"influenza-rnp-nuclear-export","display_name":"Influenza ribonucleoprotein export from the nucleus","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0c74df10-4594-5f73-a6f4-1c81d12b6eae","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:clemastanin-b-retains-influenza-rnp-in-the-nucleus-event","event_type":"observed_relationship","label":"Influenza virus ribonucleoprotein was retained in the nucleus after treatment with clemastanin B, and virus titre fell when cells were treated after infection, particularly at the early stage.","description":"Influenza virus ribonucleoprotein was retained in the nucleus after treatment with clemastanin B, and virus titre fell when cells were treated after infection, particularly at the early stage.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0526af10-74ff-50d5-9602-125327302c48","slug":"clemastanin-b","display_name":"Clemastanin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"425ae2d7-6999-5d2e-97ba-7da5295fb748","slug":"influenza-rnp-nuclear-export","display_name":"Influenza ribonucleoprotein export from the nucleus","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"17a1cd0d-3859-5c6b-a8d8-8db8cdd7151a","slug":"influenza-a-b-replication","display_name":"Influenza A and B virus replication in MDCK cells","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Treatment after infection","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"MDCK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Clemastanin B, 7S,8R,8'R-(−)-lariciresinol-4,4'-bis-O-β-D-glucopyranoside","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The authors state the result is consistent with viral endocytosis, uncoating or ribonucleoprotein export as the target and do not distinguish between them.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"MDCK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Influenza virus ribonucleoprotein was retained in the nucleus after treatment with clemastanin B, and virus titre fell when cells were treated after infection, particularly at the early stage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro. (2013). https://pubmed.ncbi.nlm.nih.gov/23403777/ DOI: 10.3892/ijmm.2013.1274","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Viral ribonucleoprotein localisation and virus titre","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"451ccaf6-c167-5781-8a5e-c4ffd8e31883","evidence_kind":"source_excerpt","locator":"Lines 132-141","start_line":132,"end_line":141,"excerpt":"## clemastanin-b-retains-influenza-rnp-in-the-nucleus\nInfluenza virus ribonucleoprotein was retained in the nucleus after treatment with clemastanin B, and virus titre fell when cells were treated after infection, particularly at the early stage.\nModel/species: MDCK cells\nTissue/system: Viral ribonucleoprotein localisation and virus titre\nExposure: Clemastanin B, 7S,8R,8'R-(−)-lariciresinol-4,4'-bis-O-β-D-glucopyranoside\nRoute: In vitro\nDuration: Treatment after infection\nLimits: The authors state the result is consistent with viral endocytosis, uncoating or ribonucleoprotein export as the target and do not distinguish between them.\nPrimary reference: Antiviral activity of Isatis indigotica root-derived clemastanin B against human and avian influenza A and B viruses in vitro. (2013). https://pubmed.ncbi.nlm.nih.gov/23403777/ DOI: 10.3892/ijmm.2013.1274\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}