{"id":"801ffb83-acd1-51aa-8e89-248b1eb78273","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-mouse-null-glycine-formate","predicate":"reduces-metabolic-flux","statement":"Isolated Slc25a32-null embryo mitochondria produced about 10.6% of control labeled formate from [13C2]-glycine over 24 hours.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c334a0bc-08a8-5148-8b68-30521bbafabd","mechanism_event_label":"The same genetic defect sharply reduced one-carbon output from glycine.","subject":{"id":"b2921ee4-204b-56e3-81c6-a049773b67a4","slug":"mouse-slc25a32-null","display_name":"Slc25a32-null mouse genotype","entity_type_key":"gene"},"object":{"id":"146f50c3-82b9-5786-b7ef-c5c8f498dccd","slug":"glycine-to-formate-flux","display_name":"Mitochondrial glycine-derived formate production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c334a0bc-08a8-5148-8b68-30521bbafabd","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-mouse-null-glycine-formate-event","event_type":"biochemical_relationship","label":"The same genetic defect sharply reduced one-carbon output from glycine.","description":"Isolated Slc25a32-null embryo mitochondria produced about 10.6% of control labeled formate from [13C2]-glycine over 24 hours.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"labeled substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"role":"measured product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e7d7cbb3-7729-537d-92fd-6fc9aaf3c24a","slug":"mouse-dld","display_name":"Mouse dihydrolipoamide dehydrogenase / Dld","entity_type_key":"protein"},"role":"impaired glycine-cleavage component","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b2921ee4-204b-56e3-81c6-a049773b67a4","slug":"mouse-slc25a32-null","display_name":"Slc25a32-null mouse genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"146f50c3-82b9-5786-b7ef-c5c8f498dccd","slug":"glycine-to-formate-flux","display_name":"Mitochondrial glycine-derived formate production","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"B2-dependent mitochondrial function supports glycine carbon entry into folate-related one-carbon supply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC11072207\", \"locator\": \"HTML article p\", \"paragraph_index\": 43, \"char_start\": 0, \"char_end\": 1215, \"evidence_access\": \"full-text\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Slc25a32-null embryos and missense knock-in mice, isolated mitochondria and skeletal-muscle enzyme assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Slc25a32-null mitochondria supplied [1,2-13C2]-glycine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Long isolated-mitochondrial assay; this is glycine-derived flux, not total cellular formate synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The same genetic defect sharply reduced one-carbon output from glycine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[peng-2022-slc25a32] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism (2022). https://pubmed.ncbi.nlm.nih.gov/35727412/ DOI: 10.1007/s00018-022-04404-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryo mitochondria, 24-hour isotope incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c83ad161-cbb9-5780-8b7c-7bea47d5ddbd","evidence_kind":"source_excerpt","locator":"Lines 888-900","start_line":888,"end_line":900,"excerpt":"### b2-met-mouse-null-glycine-formate\nIsolated Slc25a32-null embryo mitochondria produced about 10.6% of control labeled formate from [13C2]-glycine over 24 hours.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The same genetic defect sharply reduced one-carbon output from glycine.\norganism: Mus musculus\ntissue_or_cell_type: Embryo mitochondria, 24-hour isotope incubation\nexperimental_model: Slc25a32-null embryos and missense knock-in mice, isolated mitochondria and skeletal-muscle enzyme assays.\nlimitations: Long isolated-mitochondrial assay; this is glycine-derived flux, not total cellular formate synthesis.\nexposure: Slc25a32-null mitochondria supplied [1,2-13C2]-glycine.\ncross_nutrient: B2-dependent mitochondrial function supports glycine carbon entry into folate-related one-carbon supply.\nevidence_spans: [{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC11072207\", \"locator\": \"HTML article p\", \"paragraph_index\": 43, \"char_start\": 0, \"char_end\": 1215, \"evidence_access\": \"full-text\"}]\n[peng-2022-slc25a32] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism (2022). https://pubmed.ncbi.nlm.nih.gov/35727412/ DOI: 10.1007/s00018-022-04404-0","model_system":"Slc25a32-null embryos and missense knock-in mice, isolated mitochondria and skeletal-muscle enzyme assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [peng-2022-slc25a32] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism (2022). https://pubmed.ncbi.nlm.nih.gov/35727412/ DOI: 10.1007/s00018-022-04404-0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}