{"id":"7ff3d6ba-841d-5977-9ac1-dc8cb3c008e2","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-trans-zip14-zn-inhibits-mn","predicate":"inhibits","statement":"Zn(II) inhibited mouse ZIP14-mediated Mn(II) uptake in Xenopus oocytes under the tested competition conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"04640c96-322b-5ce2-ab32-05bc1f626cb4","mechanism_event_label":"Zn(II) competed with manganese entry through ZIP14 in this assay.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"ef134340-d6ae-5c8f-8b76-303bcc66760a","slug":"cellular-manganese-uptake","display_name":"Cellular manganese uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"04640c96-322b-5ce2-ab32-05bc1f626cb4","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-trans-zip14-zn-inhibits-mn-event","event_type":"biochemical_relationship","label":"Zn(II) competed with manganese entry through ZIP14 in this assay.","description":"Zn(II) inhibited mouse ZIP14-mediated Mn(II) uptake in Xenopus oocytes under the tested competition conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9e0e70ec-aa5b-584d-a6ea-28c400cb3100","slug":"mouse-slc39a14","display_name":"Mouse ZIP14 (Slc39a14)","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"inhibited_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ef134340-d6ae-5c8f-8b76-303bcc66760a","slug":"cellular-manganese-uptake","display_name":"Cellular manganese uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse ZIP14 in RNA-injected Xenopus laevis oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"2 micromolar radiolabeled Mn(II) with candidate inhibitor metal at 20 micromolar and 1 mM L-ascorbic acid; Figure 9B.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A tenfold molar competitor in a heterologous system is not a dietary competition threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse protein in Xenopus laevis oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Zn(II) competed with manganese entry through ZIP14 in this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d08a004f-81ba-5167-9268-934e48275c70","evidence_kind":"source_excerpt","locator":"Lines 318-329","start_line":318,"end_line":329,"excerpt":"### mn-trans-zip14-zn-inhibits-mn\nZn(II) inhibited mouse ZIP14-mediated Mn(II) uptake in Xenopus oocytes under the tested competition conditions.\nCondition category: normal\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Zn(II) competed with manganese entry through ZIP14 in this assay.\norganism: Mouse protein in Xenopus laevis oocytes\ntissue_or_cell_type: Oocyte plasma membrane\nexperimental_model: Mouse ZIP14 in RNA-injected Xenopus laevis oocytes\nlimitations: A tenfold molar competitor in a heterologous system is not a dietary competition threshold.\nexposure: 2 micromolar radiolabeled Mn(II) with candidate inhibitor metal at 20 micromolar and 1 mM L-ascorbic acid; Figure 9B.\ncross_nutrient: true\n[mn-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","model_system":"Mouse ZIP14 in RNA-injected Xenopus laevis oocytes","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mn-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"03224387-8a73-5b4f-906d-9f0be6625b7f","stable_key":"import-be889add-cec8-500b-be89-676431432a70","title":"Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f029ee5a1133a4f296047f06a6f0178deb9fd02e8d9707bc285bbe0f4e08fcb5","revision_id":"a77068c1-5a13-5aa9-bbac-d1cff6d34f15","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}