{"id":"7fc74ecd-6a4b-576a-b478-208e8cb25108","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-muscle-pump-loss","predicate":"maintains","statement":"Rodent potassium depletion reversibly reduced muscle ouabain-binding sites and pump-mediated potassium uptake capacity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b9d6ce8e-3dde-53ca-8077-f49080443b4a","mechanism_event_label":"Depleted muscle had fewer functional sodium-potassium pumps.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"dc51f0dd-3305-53a7-8122-8ec9e1a639da","slug":"sodium-potassium-atpase-abundance","display_name":"Sodium-potassium ATPase abundance","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b9d6ce8e-3dde-53ca-8077-f49080443b4a","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-muscle-pump-loss-event","event_type":"biochemical_relationship","label":"Depleted muscle had fewer functional sodium-potassium pumps.","description":"Rodent potassium depletion reversibly reduced muscle ouabain-binding sites and pump-mediated potassium uptake capacity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"27c3e7bd-c6e1-5344-8130-b231a9da2cfb","slug":"sodium-potassium-atpase","display_name":"Sodium-potassium ATPase complexes","entity_type_key":"protein_family"},"role":"measured transport machinery","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"coupled transported ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"uptake tracer","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"dc51f0dd-3305-53a7-8122-8ec9e1a639da","slug":"sodium-potassium-atpase-abundance","display_name":"Sodium-potassium ATPase abundance","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Potassium status alters machinery that also controls sodium transport.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat/mouse soleus and extensor digitorum longus; K-free diet, diuretic or K-binding-resin depletion models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Diet, diuretic and resin models differ; abstract does not provide each regimen duration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat and mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Depleted muscle had fewer functional sodium-potassium pumps.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[norgaard-1981-muscle] Potassium depletion decreases the number of 3H-ouabain binding sites and the active Na-K transport in skeletal muscle (1981). https://www.nature.com/articles/293739a0 DOI: 10.1038/293739a0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Soleus/extensor digitorum longus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"0fa53942-5a1f-5f0d-9252-5ebbc70c0dde","evidence_kind":"source_excerpt","locator":"Lines 739-749","start_line":739,"end_line":749,"excerpt":"### k-depletion-muscle-pump-loss\nRodent potassium depletion reversibly reduced muscle ouabain-binding sites and pump-mediated potassium uptake capacity.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Depleted muscle had fewer functional sodium-potassium pumps.\norganism: Rat and mouse\ntissue_or_cell_type: Soleus/extensor digitorum longus\nexperimental_model: Rat/mouse soleus and extensor digitorum longus; K-free diet, diuretic or K-binding-resin depletion models.\nlimitations: Diet, diuretic and resin models differ; abstract does not provide each regimen duration.\ncross_nutrient: Potassium status alters machinery that also controls sodium transport.\n[norgaard-1981-muscle] Potassium depletion decreases the number of 3H-ouabain binding sites and the active Na-K transport in skeletal muscle (1981). https://www.nature.com/articles/293739a0 DOI: 10.1038/293739a0","model_system":"Rat/mouse soleus and extensor digitorum longus; K-free diet, diuretic or K-binding-resin depletion models.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [norgaard-1981-muscle] Potassium depletion decreases the number of 3H-ouabain binding sites and the active Na-K transport in skeletal muscle (1981). https://www.nature.com/articles/293739a0 DOI: 10.1038/293739a0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}