{"id":"7fa9ca46-b7be-5a54-855a-28c86faf7bfa","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-gsta1-expression","predicate":"reduces_expression","statement":"Fisetin reduced GSTA1 mRNA and protein in proliferating human Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"53223823-faaa-56ed-880d-6e084bb8fe8e","mechanism_event_label":"Expression and direct enzyme inhibition are separate observations.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"35e312f9-e82c-5434-9357-1cfa61fe85e8","slug":"gsta1","display_name":"Human glutathione S-transferase A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"53223823-faaa-56ed-880d-6e084bb8fe8e","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-gsta1-expression-event","event_type":"observed_relationship","label":"Expression and direct enzyme inhibition are separate observations.","description":"Fisetin reduced GSTA1 mRNA and protein in proliferating human Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"35e312f9-e82c-5434-9357-1cfa61fe85e8","slug":"gsta1","display_name":"Human glutathione S-transferase A1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human colorectal adenocarcinoma cell culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell state matters; the proposed binding-site location was based on modeling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Expression and direct enzyme inhibition are separate observations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Interaction of the Flavonoid Fisetin with Human Glutathione Transferase A1-1. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33806779/ · DOI 10.3390/metabo11030190","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5d91f9a8-7191-5ed5-90a6-3684efc6b19e","evidence_kind":"source_excerpt","locator":"Lines 96-102","start_line":96,"end_line":102,"excerpt":"## fisetin-gsta1-expression\nExpression and direct enzyme inhibition are separate observations.\nFisetin reduced GSTA1 mRNA and protein in proliferating human Caco-2 cells.\nModel: Human colorectal adenocarcinoma cell culture.\nLimitations: Cell state matters; the proposed binding-site location was based on modeling.\nEvidence access: Primary abstract\nThe Interaction of the Flavonoid Fisetin with Human Glutathione Transferase A1-1. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33806779/ · DOI 10.3390/metabo11030190","model_system":"Human colorectal adenocarcinoma cell culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}