{"id":"7f899096-2f19-5f8d-bc17-bbfac66a38a5","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-human-methylation","predicate":"methylates","statement":"Ten human liver samples methylated fisetin; the reported mean activity was 183 +/- 15 pmol/min/mg and the apparent Km was 8.6 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"99c7b866-b92d-52e1-a928-02a137eb6d43","mechanism_event_label":"Human liver can chemically modify fisetin.","subject":{"id":"45ce6e62-4e41-556b-b13a-a54ccbc5fa02","slug":"human-liver-fisetin-methylation","display_name":"Human liver fisetin-methylating activity","entity_type_key":"cellular_process"},"object":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"99c7b866-b92d-52e1-a928-02a137eb6d43","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-human-methylation-event","event_type":"observed_relationship","label":"Human liver can chemically modify fisetin.","description":"Ten human liver samples methylated fisetin; the reported mean activity was 183 +/- 15 pmol/min/mg and the apparent Km was 8.6 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"45ce6e62-4e41-556b-b13a-a54ccbc5fa02","slug":"human-liver-fisetin-methylation","display_name":"Human liver fisetin-methylating activity","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9fdb2610-ab87-5de2-9658-ed83bc42b3fc","slug":"geraldol","display_name":"Geraldol / 3-prime-O-methylfisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ex vivo human liver enzyme assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract does not resolve the responsible enzyme isoform or whole-body methyl-donor demand.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human liver can chemically modify fisetin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Methylation of quercetin and fisetin, flavonoids widely distributed in edible vegetables, fruits and wine, by human liver. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12051572/ · DOI 10.5414/cpp40207","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7b4caefa-406e-57a0-bfa7-e5798d79bc58","evidence_kind":"source_excerpt","locator":"Lines 16-22","start_line":16,"end_line":22,"excerpt":"## fisetin-human-methylation\nHuman liver can chemically modify fisetin.\nTen human liver samples methylated fisetin; the reported mean activity was 183 +/- 15 pmol/min/mg and the apparent Km was 8.6 micromolar.\nModel: Ex vivo human liver enzyme assays.\nLimitations: The abstract does not resolve the responsible enzyme isoform or whole-body methyl-donor demand.\nEvidence access: Primary abstract\nMethylation of quercetin and fisetin, flavonoids widely distributed in edible vegetables, fruits and wine, by human liver. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12051572/ · DOI 10.5414/cpp40207","model_system":"Ex vivo human liver enzyme assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}