{"id":"7f86775c-9e06-54a0-a6cd-d75dc9b0a359","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-beta-oxidation","predicate":"undergoes_oxidative_metabolism","statement":"Carbon-13 NMR detected alanine-derived glutamate and aspartate in BRIN-BD11 cells; oligomycin attenuated alanine-stimulated insulin secretion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f6cfde0c-02ef-5af2-bc0f-deb47a78c775","mechanism_event_label":"Oxidative metabolism contributed to the secretory response in this cell line.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"f6ea146b-ddd3-5366-8362-b9e0b3a0928b","slug":"rat-brin-alanine-oxidation","display_name":"Alanine oxidative metabolism in rat BRIN-BD11 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f6cfde0c-02ef-5af2-bc0f-deb47a78c775","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-beta-oxidation-event","event_type":"observed_relationship","label":"Oxidative metabolism contributed to the secretory response in this cell line.","description":"Carbon-13 NMR detected alanine-derived glutamate and aspartate in BRIN-BD11 cells; 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this is not a selective proof of one alanine enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Oxidative metabolism contributed to the secretory response in this cell line.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A nuclear magnetic resonance-based demonstration of substantial oxidative L-alanine metabolism and L-alanine-enhanced glucose metabolism in a clonal pancreatic beta-cell line: metabolism of L-alanine is important to the regulation of insulin secretion. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12031957/ · DOI 10.2337/diabetes.51.6.1714","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"55a00e99-70ad-58eb-9dba-91836f26b026","evidence_kind":"source_excerpt","locator":"Lines 360-366","start_line":360,"end_line":366,"excerpt":"## alanine-beta-oxidation\nOxidative metabolism contributed to the secretory response in this cell line.\nCarbon-13 NMR detected alanine-derived glutamate and aspartate in BRIN-BD11 cells; oligomycin attenuated alanine-stimulated insulin secretion.\nModel: Rat clonal BRIN-BD11 beta-cell experiments.\nLimitations: Oligomycin broadly blocks oxidative phosphorylation; this is not a selective proof of one alanine enzyme.\nEvidence access: Primary abstract\nA nuclear magnetic resonance-based demonstration of substantial oxidative L-alanine metabolism and L-alanine-enhanced glucose metabolism in a clonal pancreatic beta-cell line: metabolism of L-alanine is important to the regulation of insulin secretion. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12031957/ · DOI 10.2337/diabetes.51.6.1714","model_system":"Rat clonal BRIN-BD11 beta-cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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