{"id":"7f1f1692-5632-560a-a2b0-bc42a2b5206e","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-bpnt2-brain-loss","predicate":"loss_changes","statement":"Nervous-system Bpnt2 deletion lowered chondroitin-4-sulfation and increased chondroitin-6-sulfation in selected brain regions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d4b5bb48-d09e-5a1d-bc06-0bf8fcb03674","mechanism_event_label":"This enzyme also changes the brain extracellular matrix.","subject":{"id":"c604ce32-6978-5132-830e-a515596db369","slug":"mouse-bpnt2","display_name":"Mouse Golgi bisphosphate nucleotidase 2 / Bpnt2","entity_type_key":"protein"},"object":{"id":"51d678fc-693b-5f4a-9d70-119a66eb90c2","slug":"mouse-brain-chondroitin-sulfation","display_name":"Mouse cortical and hippocampal chondroitin sulfation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d4b5bb48-d09e-5a1d-bc06-0bf8fcb03674","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-bpnt2-brain-loss-event","event_type":"observed_relationship","label":"This enzyme also changes the brain extracellular matrix.","description":"Nervous-system Bpnt2 deletion lowered chondroitin-4-sulfation and increased chondroitin-6-sulfation in selected brain regions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c604ce32-6978-5132-830e-a515596db369","slug":"mouse-bpnt2","display_name":"Mouse Golgi bisphosphate nucleotidase 2 / Bpnt2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"51d678fc-693b-5f4a-9d70-119a66eb90c2","slug":"mouse-brain-chondroitin-sulfation","display_name":"Mouse cortical and hippocampal chondroitin sulfation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Nestin-Cre conditional mouse knockout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No significant gross perineuronal-net or tested behavioral abnormalities were detected; not a lithium treatment experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"This enzyme also changes the brain extracellular matrix.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bisphosphate nucleotidase 2 (BPNT2), a molecular target of lithium, regulates chondroitin sulfation patterns in the cerebral cortex and hippocampus. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34920982/ · DOI 10.1016/j.jbior.2021.100858","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c5c8d399-950a-5328-9ddc-8d0c2172ae50","evidence_kind":"source_excerpt","locator":"Lines 168-174","start_line":168,"end_line":174,"excerpt":"## lithium-bpnt2-brain-loss\nThis enzyme also changes the brain extracellular matrix.\nNervous-system Bpnt2 deletion lowered chondroitin-4-sulfation and increased chondroitin-6-sulfation in selected brain regions.\nModel: Nestin-Cre conditional mouse knockout.\nLimitations: No significant gross perineuronal-net or tested behavioral abnormalities were detected; not a lithium treatment experiment.\nEvidence access: Primary abstract\nBisphosphate nucleotidase 2 (BPNT2), a molecular target of lithium, regulates chondroitin sulfation patterns in the cerebral cortex and hippocampus. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34920982/ · DOI 10.1016/j.jbior.2021.100858","model_system":"Nestin-Cre conditional mouse knockout.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"dc67e965ba78458cf2d88d000d687641d9104354115ab16529a3a64941bd777d","revision_id":"4af127bf-9ccc-516b-9481-fafd453c2c35","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}