{"id":"7eccdd27-7d86-577a-9db4-1af6dfae2844","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-marc2-nitrite","predicate":"reduces","statement":"Reduced human mARC2 also catalyzed nitrite-to-NO conversion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"012fe405-f5c2-5728-9485-dca9d4a08db4","mechanism_event_label":"mARC2 shares this conditional NO-forming capability.","subject":{"id":"c3c9e014-7930-5cfc-b7ce-a142dcdb3dd0","slug":"mtarc2","display_name":"Human mitochondrial amidoxime-reducing component 2 / MTARC2","entity_type_key":"protein"},"object":{"id":"03ffed32-aa18-5d63-96a4-8a7ebedaf7a9","slug":"nitrite","display_name":"Nitrite / NO2(-)","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"012fe405-f5c2-5728-9485-dca9d4a08db4","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-marc2-nitrite-event","event_type":"biochemical_relationship","label":"mARC2 shares this conditional NO-forming capability.","description":"Reduced human mARC2 also catalyzed nitrite-to-NO conversion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"electron donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c3c9e014-7930-5cfc-b7ce-a142dcdb3dd0","slug":"mtarc2","display_name":"Human mitochondrial amidoxime-reducing component 2 / MTARC2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"03ffed32-aa18-5d63-96a4-8a7ebedaf7a9","slug":"nitrite","display_name":"Nitrite / NO2(-)","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/24500710.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e4c2ab41c945d420266290d0241752039d7de2cb6223f9c64c7d0525c5688d9b\", \"start_char\": 0, \"end_char\": 1585, \"text_sha256\": \"e4c2ab41c945d420266290d0241752039d7de2cb6223f9c64c7d0525c5688d9b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human mARC1/mARC2, active-site mutation, tungsten replacement and HEK expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Reduced enzyme, nitrite, NADH/CYB5/CYB5R; pH 7.5 versus 6.5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Shows nitrite-reducing capacity; its share of NO production in normal people and response to mineral intake are not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens proteins and HEK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"mARC2 shares this conditional NO-forming capability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p24500710] Nitrite reductase and nitric-oxide synthase activity of the mitochondrial molybdopterin enzymes mARC1 and mARC2. (2014). https://pubmed.ncbi.nlm.nih.gov/24500710/ DOI: 10.1074/jbc.m114.555177","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified redox system and human cell model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"78fd75f0-a002-516c-8879-b5ce29299b56","evidence_kind":"source_excerpt","locator":"Lines 1041-1052","start_line":1041,"end_line":1052,"excerpt":"### mo-marc2-nitrite\nReduced human mARC2 also catalyzed nitrite-to-NO conversion.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: mARC2 shares this conditional NO-forming capability.\norganism: Homo sapiens proteins and HEK cells\ntissue_or_cell_type: Purified redox system and human cell model\nexperimental_model: Recombinant human mARC1/mARC2, active-site mutation, tungsten replacement and HEK expression\nlimitations: Shows nitrite-reducing capacity; its share of NO production in normal people and response to mineral intake are not established.\nexposure: Reduced enzyme, nitrite, NADH/CYB5/CYB5R; pH 7.5 versus 6.5\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/24500710.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e4c2ab41c945d420266290d0241752039d7de2cb6223f9c64c7d0525c5688d9b\", \"start_char\": 0, \"end_char\": 1585, \"text_sha256\": \"e4c2ab41c945d420266290d0241752039d7de2cb6223f9c64c7d0525c5688d9b\"}\n[mo-p24500710] Nitrite reductase and nitric-oxide synthase activity of the mitochondrial molybdopterin enzymes mARC1 and mARC2. 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