{"id":"7ec7a76c-ca71-5c6b-ba30-e1f522773ffa","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nad-timecourse","predicate":"changes_over_time","statement":"In human HeLa cells, resveratrol initially reduced NAD with increased nicotinamide/ADP-ribose at 15 minutes; low concentrations raised NAD by one hour.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4b31fa35-da42-5ffd-a53d-5317576e3ded","mechanism_event_label":"Initial consumption and later replenishment are separate phases.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"07ca7f07-7da8-5fb0-bb3e-11ebca34ccd1","slug":"human-hela-nad-content","display_name":"NAD content in human HeLa cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4b31fa35-da42-5ffd-a53d-5317576e3ded","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nad-timecourse-event","event_type":"observed_relationship","label":"Initial consumption and later replenishment are separate phases.","description":"In human HeLa cells, resveratrol initially reduced NAD with increased nicotinamide/ADP-ribose at 15 minutes; 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5 micromolar used in dependency experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Whole-cell NAD is not a measurement of every subcellular pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Initial consumption and later replenishment are separate phases.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A human tRNA synthetase is a potent PARP1-activating effector target for resveratrol. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25533949/ · DOI 10.1038/nature14028","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ec6f9218-64fa-55cf-baa6-7b5fa8123df5","evidence_kind":"source_excerpt","locator":"Lines 310-316","start_line":310,"end_line":316,"excerpt":"## resveratrol-nad-timecourse\nInitial consumption and later replenishment are separate phases.\nIn human HeLa cells, resveratrol initially reduced NAD with increased nicotinamide/ADP-ribose at 15 minutes; low concentrations raised NAD by one hour.\nModel: Low-dose time course; 5 micromolar used in dependency experiments.\nLimitations: Whole-cell NAD is not a measurement of every subcellular pool.\nEvidence access: Primary full text\nA human tRNA synthetase is a potent PARP1-activating effector target for resveratrol. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25533949/ · DOI 10.1038/nature14028","model_system":"Low-dose time course; 5 micromolar used in dependency experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"04885b4bee72f912a7f3f618f1b2bf79c944dbcb20500398c2729fc8228dcd9a","revision_id":"99504ff2-f1d3-5f4c-bc31-e1030d27a918","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}