{"id":"7ea3d0dd-926c-52b8-bad4-5548516ec635","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-pdxk-pm","predicate":"phosphorylates","statement":"Purified recombinant human PDXK phosphorylated pyridoxamine to its corresponding 5-prime phosphate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"450ea5bf-2a8e-5323-ba05-efd3f22e62c3","mechanism_event_label":"PDXK adds phosphate to this B6 precursor.","subject":{"id":"d2d46db7-13a4-595f-adaa-70ad6ca31a6c","slug":"pdxk","display_name":"Pyridoxal kinase / PDXK","entity_type_key":"protein"},"object":{"id":"9c195b42-dc87-51f6-a317-d4585a0e8d84","slug":"pyridoxamine","display_name":"Pyridoxamine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"450ea5bf-2a8e-5323-ba05-efd3f22e62c3","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-pdxk-pm-event","event_type":"biochemical_relationship","label":"PDXK adds phosphate to this B6 precursor.","description":"Purified recombinant human PDXK phosphorylated pyridoxamine to its corresponding 5-prime phosphate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9c195b42-dc87-51f6-a317-d4585a0e8d84","slug":"pyridoxamine","display_name":"Pyridoxamine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"phosphate donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c8dec85c-c85d-5a55-ba85-ad3c1b3e332b","slug":"pyridoxamine-phosphate","display_name":"Pyridoxamine 5-prime-phosphate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"136c3764-1b93-5f79-8673-9001cab9bc3d","slug":"adp","display_name":"Adenosine diphosphate","entity_type_key":"small_molecule"},"role":"nucleotide product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d2d46db7-13a4-595f-adaa-70ad6ca31a6c","slug":"pdxk","display_name":"Pyridoxal kinase / PDXK","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Indexed abstract and publisher abstract; human enzyme findings only","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant human and E. coli pyridoxal kinases.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified-enzyme substrate kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Model-specific evidence; no dietary threshold or treatment benefit established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PDXK adds phosphate to this B6 precursor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[disalvo2004] Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases. (2004). https://pubmed.ncbi.nlm.nih.gov/15249053/ DOI: 10.1016/j.pep.2004.04.021","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified human enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"044e2dcd-6aff-5fc7-8bc4-34872ac1561d","evidence_kind":"source_excerpt","locator":"Lines 176-187","start_line":176,"end_line":187,"excerpt":"### b6-transport-pdxk-pm\nPurified recombinant human PDXK phosphorylated pyridoxamine to its corresponding 5-prime phosphate.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: PDXK adds phosphate to this B6 precursor.\norganism: Homo sapiens\ntissue_or_cell_type: Purified human enzyme\nexperimental_model: Purified recombinant human and E. coli pyridoxal kinases.\nlimitations: Model-specific evidence; no dietary threshold or treatment benefit established.\nexposure: Purified-enzyme substrate kinetics.\nevidence_location: Indexed abstract and publisher abstract; human enzyme findings only\n[disalvo2004] Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases. 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