{"id":"7e627541-87e1-5903-8ab4-eec985764a70","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-slc26a9-membranes","predicate":"loss_of_function_impairs","statement":"Slc26a9 deletion eliminated parietal-cell tubulovesicles and shifted H-K-ATPase localization to the apical pole.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6f56158b-73b4-5496-aac4-814463e88ce7","mechanism_event_label":"The secretory machinery changed as well as the acid output.","subject":{"id":"fc19b17b-1081-574e-be02-53f75d2ab9a9","slug":"mouse-slc26a9","display_name":"Mouse gastric anion transporter Slc26a9","entity_type_key":"protein"},"object":{"id":"cc6fd380-3885-5729-a151-34d3b0495da3","slug":"parietal-secretory-membrane-organization","display_name":"Gastric parietal-cell secretory membrane organization","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6f56158b-73b4-5496-aac4-814463e88ce7","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-slc26a9-membranes-event","event_type":"biochemical_relationship","label":"The secretory machinery changed as well as the acid output.","description":"Slc26a9 deletion eliminated parietal-cell tubulovesicles and shifted H-K-ATPase localization to the apical pole.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fc19b17b-1081-574e-be02-53f75d2ab9a9","slug":"mouse-slc26a9","display_name":"Mouse gastric anion transporter Slc26a9","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cc6fd380-3885-5729-a151-34d3b0495da3","slug":"parietal-secretory-membrane-organization","display_name":"Gastric parietal-cell secretory membrane organization","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/19004773.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ed1a2033c883ec9b4eeab6a9c0bae03d1f478adf66913c3d5e8c43879a836d3e\", \"start_char\": 0, \"end_char\": 1342, \"text_sha256\": \"ed1a2033c883ec9b4eeab6a9c0bae03d1f478adf66913c3d5e8c43879a836d3e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Slc26a9 deletion and heterologous transport assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Knockout at five weeks; expression assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different assays showed conductance or exchange; do not assign one universal stoichiometry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse protein; oocytes and cultured cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The secretory machinery changed as well as the acid output.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p19004773] Deletion of the chloride transporter Slc26a9 causes loss of tubulovesicles in parietal cells and impairs acid secretion in the stomach. (2008). https://pubmed.ncbi.nlm.nih.gov/19004773/ DOI: 10.1073/pnas.0800616105","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Gastric parietal cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"708bdf87-f643-5f39-9867-468c700d7572","evidence_kind":"source_excerpt","locator":"Lines 211-222","start_line":211,"end_line":222,"excerpt":"### chloride-slc26a9-membranes\nSlc26a9 deletion eliminated parietal-cell tubulovesicles and shifted H-K-ATPase localization to the apical pole.\nCondition category: machinery_impairment\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The secretory machinery changed as well as the acid output.\norganism: Mouse protein; oocytes and cultured cells\ntissue_or_cell_type: Gastric parietal cells\nexperimental_model: Slc26a9 deletion and heterologous transport assays\nlimitations: Different assays showed conductance or exchange; do not assign one universal stoichiometry.\nexposure: Knockout at five weeks; expression assays\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/19004773.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"ed1a2033c883ec9b4eeab6a9c0bae03d1f478adf66913c3d5e8c43879a836d3e\", \"start_char\": 0, \"end_char\": 1342, \"text_sha256\": \"ed1a2033c883ec9b4eeab6a9c0bae03d1f478adf66913c3d5e8c43879a836d3e\"}\n[chloride-p19004773] Deletion of the chloride transporter Slc26a9 causes loss of tubulovesicles in parietal cells and impairs acid secretion in the stomach. (2008). https://pubmed.ncbi.nlm.nih.gov/19004773/ DOI: 10.1073/pnas.0800616105","model_system":"Slc26a9 deletion and heterologous transport assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chloride-p19004773] Deletion of the chloride transporter Slc26a9 causes loss of tubulovesicles in parietal cells and impairs acid secretion in the stomach. (2008). https://pubmed.ncbi.nlm.nih.gov/19004773/ DOI: 10.1073/pnas.0800616105","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1c2070d5-9f15-5945-b325-d0b49a32c04b","stable_key":"import-4df6a932-097e-563a-91fe-5dceec471ffd","title":"Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e32e796f293a3287c6b8f4394881db94cc741ba96e3166e44da2dd14f208f645","revision_id":"8d5fd8ef-db54-5198-a368-23329a02d81b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}