{"id":"7e5cf07f-481d-5698-af3b-a45ee4940913","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmab-adenosylates-cob-i","predicate":"adenosylates","statement":"Purified human MMAB uses ATP to adenosylate cob(I)alamin to adenosylcobalamin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b9cd4949-c9e5-513d-a9c8-eeb57471d05b","mechanism_event_label":"In a purified human-protein assay, MMAB made the B12 cofactor used by MMUT.","subject":{"id":"38f7c5d6-7610-5432-accb-fc06ba7b259f","slug":"mmab","display_name":"Human ATP:cob(I)alamin adenosyltransferase / MMAB","entity_type_key":"protein"},"object":{"id":"227597d2-ecb7-5a01-8acc-fa6a2ff5d9ff","slug":"cob-i-alamin","display_name":"Cob(I)alamin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b9cd4949-c9e5-513d-a9c8-eeb57471d05b","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmab-adenosylates-cob-i-event","event_type":"biochemical_relationship","label":"In a purified human-protein assay, MMAB made the B12 cofactor used by MMUT.","description":"Purified human MMAB uses ATP to adenosylate cob(I)alamin to adenosylcobalamin.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"adenosyl-donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"07657685-74aa-5d99-8625-0f6a3b521f07","slug":"adenosylcobalamin","display_name":"5'-Deoxyadenosylcobalamin","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38f7c5d6-7610-5432-accb-fc06ba7b259f","slug":"mmab","display_name":"Human ATP:cob(I)alamin adenosyltransferase / MMAB","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"227597d2-ecb7-5a01-8acc-fa6a2ff5d9ff","slug":"cob-i-alamin","display_name":"Cob(I)alamin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Indexed primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cob(I)alamin and ATP","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical reconstitution; does not determine cellular rate or dietary requirement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In a purified human-protein assay, MMAB made the B12 cofactor used by MMUT.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[leal-2004-human-mmab] Human ATP:Cob(I)alamin adenosyltransferase and its interaction with methionine synthase reductase. (2004). https://pubmed.ncbi.nlm.nih.gov/15347655/ DOI: 10.1074/jbc.m405449200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"efae8f44-ee13-5cbb-ab3a-d3050dbb5f0f","evidence_kind":"source_excerpt","locator":"Lines 977-989","start_line":977,"end_line":989,"excerpt":"### mmab-adenosylates-cob-i\nPurified human MMAB uses ATP to adenosylate cob(I)alamin to adenosylcobalamin.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In a purified human-protein assay, MMAB made the B12 cofactor used by MMUT.\norganism: Homo sapiens\ntissue_or_cell_type: Purified protein assay\nexperimental_model: Purified human proteins\nlimitations: Biochemical reconstitution; does not determine cellular rate or dietary requirement.\nexposure: Cob(I)alamin and ATP\ncross_nutrient: false\nevidence_location: Indexed primary abstract\n[leal-2004-human-mmab] Human ATP:Cob(I)alamin adenosyltransferase and its interaction with methionine synthase reductase. (2004). https://pubmed.ncbi.nlm.nih.gov/15347655/ DOI: 10.1074/jbc.m405449200","model_system":"Purified human proteins","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [leal-2004-human-mmab] Human ATP:Cob(I)alamin adenosyltransferase and its interaction with methionine synthase reductase. (2004). https://pubmed.ncbi.nlm.nih.gov/15347655/ DOI: 10.1074/jbc.m405449200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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