{"id":"7e5183f5-fe7a-5744-8ca9-81d7a09d7009","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyt-ion-cooperativity","predicate":"supports","statement":"GlyT1 and GlyT2 maintained forward transport under the tested elevated intracellular sodium or chloride conditions, consistent with cooperative ion/substrate binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2b5956af-b8b3-5154-b32b-36c7b57eb3ea","mechanism_event_label":"Raising one intracellular ion did not simply switch these transporters off.","subject":{"id":"39cd117a-7e5f-573f-a460-0bb8cb2b5b18","slug":"human-glyt-ion-substrate-coupling","display_name":"Ion/substrate coupling in human GlyT kinetic assays","entity_type_key":"cellular_process"},"object":{"id":"c59d9f46-6c85-5fb6-8ac6-610d2102d862","slug":"human-glyt-forward-transport","display_name":"Forward glycine transport in human GlyT assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2b5956af-b8b3-5154-b32b-36c7b57eb3ea","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyt-ion-cooperativity-event","event_type":"observed_relationship","label":"Raising one intracellular ion did not simply switch these transporters off.","description":"GlyT1 and GlyT2 maintained forward transport under the tested elevated intracellular sodium or chloride conditions, consistent with cooperative ion/substrate binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"39cd117a-7e5f-573f-a460-0bb8cb2b5b18","slug":"human-glyt-ion-substrate-coupling","display_name":"Ion/substrate coupling in human GlyT kinetic assays","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c59d9f46-6c85-5fb6-8ac6-610d2102d862","slug":"human-glyt-forward-transport","display_name":"Forward glycine transport in human GlyT assays","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f9b4b084-aace-5bd4-a98b-f41923bf4923","slug":"slc6a9","display_name":"Human glycine transporter 1 / SLC6A9","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"705fbb31-1281-5bdd-91e1-65b434ab57a9","slug":"slc6a5","display_name":"Human glycine transporter 2 / SLC6A5","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human GlyT1/GlyT2 voltage-clamp measurements and kinetic models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding-order models are interpretations constrained by currents, not direct structures of every transport state.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; 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