{"id":"7dc54f6d-bb49-5bde-91e0-89fbd00b57cd","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-microbial-human-dependence","predicate":"decreases_during","statement":"Seven days of broad-spectrum antibiotics in fifteen subjects markedly reduced circulating PAGln, with recovery after microbial repopulation.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ba478285-2381-57c3-8de0-83219e43a9db","mechanism_event_label":"The human circulating metabolite depends in part on gut microbial activity.","subject":{"id":"8968a92b-40e9-56fe-8d76-d8776750dbd6","slug":"phenylacetylglutamine","display_name":"Phenylacetylglutamine / PAGln","entity_type_key":"small_molecule"},"object":{"id":"c959e234-cc57-564f-929f-e3fb56a88286","slug":"human-antibiotic-pagln-response","display_name":"Human PAGln response to microbiota suppression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ba478285-2381-57c3-8de0-83219e43a9db","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-microbial-human-dependence-event","event_type":"observed_relationship","label":"The human circulating metabolite depends in part on gut microbial activity.","description":"Seven days of broad-spectrum antibiotics in fifteen subjects markedly reduced circulating PAGln, with recovery after microbial repopulation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8968a92b-40e9-56fe-8d76-d8776750dbd6","slug":"phenylacetylglutamine","display_name":"Phenylacetylglutamine / PAGln","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c959e234-cc57-564f-929f-e3fb56a88286","slug":"human-antibiotic-pagln-response","display_name":"Human PAGln response to microbiota suppression","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human antibiotic suppression and post-antibiotic recovery sampling at least three weeks later.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Antibiotics affect many microbes and metabolites; this does not isolate one bacterial enzyme or recommend antimicrobial use.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The human circulating metabolite depends in part on gut microbial activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"27d2e858-bd97-5c6e-97f7-c952c6589ce5","evidence_kind":"source_excerpt","locator":"Lines 366-372","start_line":366,"end_line":372,"excerpt":"## l-phenylalanine-microbial-human-dependence\nThe human circulating metabolite depends in part on gut microbial activity.\nSeven days of broad-spectrum antibiotics in fifteen subjects markedly reduced circulating PAGln, with recovery after microbial repopulation.\nModel: Human antibiotic suppression and post-antibiotic recovery sampling at least three weeks later.\nLimitations: Antibiotics affect many microbes and metabolites; this does not isolate one bacterial enzyme or recommend antimicrobial use.\nEvidence access: Primary full text\nA Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","model_system":"Human antibiotic suppression and post-antibiotic recovery sampling at least three weeks later.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4d53adcd4acc7a9aecf44ccb2623ec540c9d221a0f7c00002585870b39b8ffb8","revision_id":"fd182a81-4ab7-559a-9c77-fb4cf72982ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}