{"id":"7d805248-659d-507a-be0c-cd1aebf2ba1f","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:2opp-larval-hyperactivity","predicate":"increases","statement":"Exposure to 10 mM 2-OPP increased zebrafish larval hyperactivity.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"567df1f2-574d-5c23-973e-659c462cd6d9","mechanism_event_label":"A disease-associated metabolite affected an animal behavior assay.","subject":{"id":"80400fee-4e65-5cf7-a5a6-1cd3f93ef1ac","slug":"2-opp","display_name":"2S,6S-/2S,6R-6-(2-Oxopropyl)piperidine-2-carboxylate","entity_type_key":"small_molecule"},"object":{"id":"cf807b54-8e84-5a01-9877-c8f011ab9d8c","slug":"larval-hyperactivity","display_name":"Larval locomotor hyperactivity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"567df1f2-574d-5c23-973e-659c462cd6d9","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:2opp-larval-hyperactivity-event","event_type":"observed_intervention","label":"A disease-associated metabolite affected an animal behavior assay.","description":"Exposure to 10 mM 2-OPP increased zebrafish larval hyperactivity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80400fee-4e65-5cf7-a5a6-1cd3f93ef1ac","slug":"2-opp","display_name":"2S,6S-/2S,6R-6-(2-Oxopropyl)piperidine-2-carboxylate","entity_type_key":"small_molecule"},"role":"experimental_exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cf807b54-8e84-5a01-9877-c8f011ab9d8c","slug":"larval-hyperactivity","display_name":"Larval locomotor hyperactivity","entity_type_key":"cellular_process"},"role":"outcome","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Wild-type Tüpfel long fin zebrafish F4 larvae exposed to 10 mM 2-OPP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Classic convulsions were absent; this does not prove human seizure causation. This exposure arm did not have ALDH7A1 impairment; it is not a genetic-deficiency experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Danio rerio","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A disease-associated metabolite affected an animal behavior assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[engelke2021] Untargeted metabolomics and infrared ion spectroscopy identify biomarkers for pyridoxine-dependent epilepsy (2021). https://www.jci.org/articles/view/148272 DOI: 10.1172/JCI148272","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Whole larvae","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"47337454-6680-5a8b-81f7-6b373427a784","evidence_kind":"source_excerpt","locator":"Lines 314-322","start_line":314,"end_line":322,"excerpt":"### 2opp-larval-hyperactivity\nExposure to 10 mM 2-OPP increased zebrafish larval hyperactivity.\nPlain language: A disease-associated metabolite affected an animal behavior assay.\nCondition category: normal\norganism: Danio rerio\ntissue_or_cell_type: Whole larvae\nexperimental_model: Wild-type Tüpfel long fin zebrafish F4 larvae exposed to 10 mM 2-OPP.\nlimitations: Classic convulsions were absent; this does not prove human seizure causation. This exposure arm did not have ALDH7A1 impairment; it is not a genetic-deficiency experiment.\n[engelke2021] Untargeted metabolomics and infrared ion spectroscopy identify biomarkers for pyridoxine-dependent epilepsy (2021). https://www.jci.org/articles/view/148272 DOI: 10.1172/JCI148272","model_system":"Wild-type Tüpfel long fin zebrafish F4 larvae exposed to 10 mM 2-OPP.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [engelke2021] Untargeted metabolomics and infrared ion spectroscopy identify biomarkers for pyridoxine-dependent epilepsy (2021). https://www.jci.org/articles/view/148272 DOI: 10.1172/JCI148272","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}