{"id":"7d7ef15c-1d05-51a2-a0fb-78bf744f0030","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-serrs-protein-route","predicate":"loads_serine_onto","statement":"Human cytosolic SerRS attached L-serine to tRNA Ser and showed only a modest preference for it over tRNA Sec in the tested binding and serylation experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"76203795-98a7-5543-bb54-e8044c91cc8c","mechanism_event_label":"Serine must be attached to a tRNA before it can enter a growing protein.","subject":{"id":"990f63d9-f73d-56e3-af41-1e2569702480","slug":"sars1","display_name":"Human cytosolic seryl-tRNA synthetase / SARS1","entity_type_key":"protein"},"object":{"id":"ef285cfe-f652-55b8-95eb-1ccdfe9574cd","slug":"human-trna-ser","display_name":"Human cytosolic tRNA Ser","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"76203795-98a7-5543-bb54-e8044c91cc8c","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-serrs-protein-route-event","event_type":"observed_relationship","label":"Serine must be attached to a tRNA before it can enter a growing protein.","description":"Human cytosolic SerRS attached L-serine to tRNA Ser and showed only a modest preference for it over tRNA Sec in the tested binding and serylation experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"990f63d9-f73d-56e3-af41-1e2569702480","slug":"sars1","display_name":"Human cytosolic seryl-tRNA synthetase / SARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ef285cfe-f652-55b8-95eb-1ccdfe9574cd","slug":"human-trna-ser","display_name":"Human cytosolic tRNA Ser","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SerRS with two tRNA species and ten mutant/chimeric constructs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Carrier recognition is distinct from ordinary amino-acid transport or downstream protein phosphorylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Serine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Serine must be attached to a tRNA before it can enter a growing protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Insights into substrate promiscuity of human seryl-tRNA synthetase. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28808125/ · DOI 10.1261/rna.061069.117","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"95fce7f7-429f-594b-b083-47e8a95f69fc","evidence_kind":"source_excerpt","locator":"Lines 446-452","start_line":446,"end_line":452,"excerpt":"## l-serine-serrs-protein-route\nSerine must be attached to a tRNA before it can enter a growing protein.\nHuman cytosolic SerRS attached L-serine to tRNA Ser and showed only a modest preference for it over tRNA Sec in the tested binding and serylation experiments.\nModel: Human SerRS with two tRNA species and ten mutant/chimeric constructs.\nLimitations: Carrier recognition is distinct from ordinary amino-acid transport or downstream protein phosphorylation.\nEvidence access: Primary abstract\nInsights into substrate promiscuity of human seryl-tRNA synthetase. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28808125/ · DOI 10.1261/rna.061069.117","model_system":"Human SerRS with two tRNA species and ten mutant/chimeric constructs.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70bcf57a-36bb-563f-9073-2d616e5f155a","stable_key":"import-649e861b-265a-5912-bc36-3a73e53ef892","title":"L-Serine: synthesis, one-carbon metabolism, lipids and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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