{"id":"7d6a70e5-3386-5df1-a7d0-6dbb5aebb8ac","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-breast-cth-cbs-context","predicate":"changes_tested","statement":"The study reported opposite CTH and CBS expression responses between the two breast cancer cell lines.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9f2d6e4f-afae-5f09-ad24-795adce5fcb5","mechanism_event_label":"The cellular background changed the direction of the response.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"f4feb4b1-c8d7-54eb-902d-5769aa7b3e02","slug":"human-sac-breast-sulfur-expression","display_name":"Cell-line-dependent CTH/CBS expression response to SAC","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9f2d6e4f-afae-5f09-ad24-795adce5fcb5","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-breast-cth-cbs-context-event","event_type":"observed_relationship","label":"The cellular background changed the direction of the response.","description":"The study reported opposite CTH and CBS expression responses between the two breast cancer cell lines.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f4feb4b1-c8d7-54eb-902d-5769aa7b3e02","slug":"human-sac-breast-sulfur-expression","display_name":"Cell-line-dependent CTH/CBS expression response to SAC","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4014ee3a-0e44-5379-b541-174aa75bcf90","slug":"cth","display_name":"Human cystathionine gamma-lyase / CTH","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4688e32c-d7b6-5fca-9eb2-23307c29ae07","slug":"cbs","display_name":"Human cystathionine beta-synthase / CBS","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"2.24, 3.37 or 4.50 mM SAC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"2, 4, 6, 8 or 24 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC versus untreated cell controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"MCF-7 and MDA-MB-231 breast adenocarcinoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract does not resolve every time/dose direction; these must be retrieved before assigning a universal signed enzyme edge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cellular background changed the direction of the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[38397425] S-Allyl-L-Cysteine Affects Cell Proliferation and Expression of H2S-Synthetizing Enzymes in MCF-7 and MDA-MB-231 Adenocarcinoma Cell Lines. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38397425/ · DOI 10.3390/biom14020188","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"MCF-7 and MDA-MB-231 breast adenocarcinoma cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a58ce48f-9613-5b6d-96b8-5d66b18b7676","evidence_kind":"source_excerpt","locator":"Lines 302-309","start_line":302,"end_line":309,"excerpt":"## s-allylcysteine-breast-cth-cbs-context\nThe cellular background changed the direction of the response.\nThe study reported opposite CTH and CBS expression responses between the two breast cancer cell lines.\nModel: MCF-7 and MDA-MB-231 breast adenocarcinoma cells\nLimitations: Abstract does not resolve every time/dose direction; these must be retrieved before assigning a universal signed enzyme edge.\nEvidence access: Primary abstract\n[38397425] S-Allyl-L-Cysteine Affects Cell Proliferation and Expression of H2S-Synthetizing Enzymes in MCF-7 and MDA-MB-231 Adenocarcinoma Cell Lines. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38397425/ · DOI 10.3390/biom14020188\nStructured context: {\"organism\": \"Homo sapiens\", \"tissue_or_cell_type\": \"MCF-7 and MDA-MB-231 breast adenocarcinoma cells\", \"dose\": \"2.24, 3.37 or 4.50 mM SAC\", \"duration\": \"2, 4, 6, 8 or 24 hours\", \"route\": \"Cell culture\", \"experimental_comparison\": \"SAC versus untreated cell controls\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"MCF-7 and MDA-MB-231 breast adenocarcinoma cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"91d7aeac82e42ce559d13dd8a2e0165d37760f7f555dd01c80e0604fa6e71ea2","revision_id":"545e497c-e5e3-543c-b26b-b297cbc7bbec","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}