{"id":"7d5323cc-5b95-5ca4-8684-f25db31960c6","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-chromosome-segregation","predicate":"increases","statement":"Fisetin increased chromosome missegregation and kinetochore-positive micronuclei in TK6 and HL60 cells; breakage-related micronuclei appeared at higher tested concentrations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"cd2f50f0-9eaf-58a2-b3ec-9636e13dd7fa","mechanism_event_label":"Cell-culture toxicity included chromosome loss and breakage.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"1a203106-7fb6-5510-b237-dea4a1bc2183","slug":"human-tk6-hl60-chromosome-missegregation","display_name":"Chromosome missegregation in human TK6 and HL60 cultures","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cd2f50f0-9eaf-58a2-b3ec-9636e13dd7fa","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-chromosome-segregation-event","event_type":"observed_relationship","label":"Cell-culture toxicity included chromosome loss and breakage.","description":"Fisetin increased chromosome missegregation and kinetochore-positive micronuclei in TK6 and HL60 cells; breakage-related micronuclei appeared at higher tested concentrations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1a203106-7fb6-5510-b237-dea4a1bc2183","slug":"human-tk6-hl60-chromosome-missegregation","display_name":"Chromosome missegregation in human TK6 and HL60 cultures","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cultured-cell cytokinesis-block/CREST assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Exposure-specific hazard characterization, not demonstrated human cancer risk.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cell-culture toxicity included chromosome loss and breakage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Chromosomal malsegregation and micronucleus induction in vitro by the DNA topoisomerase II inhibitor fisetin. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781213/ · DOI 10.1016/j.mrgentox.2005.01.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a28d7e51-1aef-51a5-a831-49032bc97185","evidence_kind":"source_excerpt","locator":"Lines 488-494","start_line":488,"end_line":494,"excerpt":"## fisetin-chromosome-segregation\nCell-culture toxicity included chromosome loss and breakage.\nFisetin increased chromosome missegregation and kinetochore-positive micronuclei in TK6 and HL60 cells; breakage-related micronuclei appeared at higher tested concentrations.\nModel: Human cultured-cell cytokinesis-block/CREST assays.\nLimitations: Exposure-specific hazard characterization, not demonstrated human cancer risk.\nEvidence access: Primary abstract\nChromosomal malsegregation and micronucleus induction in vitro by the DNA topoisomerase II inhibitor fisetin. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781213/ · DOI 10.1016/j.mrgentox.2005.01.002","model_system":"Human cultured-cell cytokinesis-block/CREST assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}