{"id":"7d288016-8c6b-56d2-9dba-00412f460f2a","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-low-status-blood-dna-uracil","predicate":"low_status_associated_with_higher_dna_uracil","statement":"Folate-deficient participants had roughly eightfold higher measured blood DNA uracil than controls in the 1997 study.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"17c7515b-3586-52f8-a008-99a758ba7b45","mechanism_event_label":"Insufficient folate was associated with more of an inappropriate DNA base in the measured samples.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"2bdb9a6d-0a55-59ef-98ce-51168d23d870","slug":"blood-dna-uracil-content","display_name":"Uracil content of blood-cell DNA","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"17c7515b-3586-52f8-a008-99a758ba7b45","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-low-status-blood-dna-uracil-event","event_type":"observed_intervention","label":"Insufficient folate was associated with more of an inappropriate DNA base in the measured samples.","description":"Folate-deficient participants had roughly eightfold higher measured blood DNA uracil than controls in the 1997 study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2bdb9a6d-0a55-59ef-98ce-51168d23d870","slug":"blood-dna-uracil-content","display_name":"Uracil content of blood-cell DNA","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Selected splenectomized human volunteers: 22 at baseline, 19 supplementation completers; additional blood/marrow samples and Crohn case analyzed separately.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Folic acid 5 mg/day for eight weeks; blood DNA uracil and erythrocyte/reticulocyte micronuclei.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Enriched selection by micronucleus frequency and older assay methodology limit extrapolation. The full repair-to-break sequence was a mechanistic interpretation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Insufficient folate was associated with more of an inappropriate DNA base in the measured samples.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fol-blount1997] Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: implications for cancer and neuronal damage (1997). https://pubmed.ncbi.nlm.nih.gov/9096386/ DOI: 10.1073/pnas.94.7.3290","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human blood-cell DNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ab1f5385-73c3-5c36-b35b-7dc18e31565e","evidence_kind":"source_excerpt","locator":"Lines 1469-1479","start_line":1469,"end_line":1479,"excerpt":"### fol-low-status-blood-dna-uracil\nFolate-deficient participants had roughly eightfold higher measured blood DNA uracil than controls in the 1997 study.\nCondition category: nutrient_deficiency\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Insufficient folate was associated with more of an inappropriate DNA base in the measured samples.\norganism: Homo sapiens\ntissue_or_cell_type: Human blood-cell DNA\nexperimental_model: Selected splenectomized human volunteers: 22 at baseline, 19 supplementation completers; additional blood/marrow samples and Crohn case analyzed separately.\nlimitations: Enriched selection by micronucleus frequency and older assay methodology limit extrapolation. The full repair-to-break sequence was a mechanistic interpretation.\nexposure: Folic acid 5 mg/day for eight weeks; blood DNA uracil and erythrocyte/reticulocyte micronuclei.\n[fol-blount1997] Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: implications for cancer and neuronal damage (1997). https://pubmed.ncbi.nlm.nih.gov/9096386/ DOI: 10.1073/pnas.94.7.3290","model_system":"Selected splenectomized human volunteers: 22 at baseline, 19 supplementation completers; additional blood/marrow samples and Crohn case analyzed separately.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fol-blount1997] Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: implications for cancer and neuronal damage (1997). https://pubmed.ncbi.nlm.nih.gov/9096386/ DOI: 10.1073/pnas.94.7.3290","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}