{"id":"7d26ced7-b89a-58d7-86e7-a8524f36a205","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:plp-alliinase","predicate":"reported_relationship","statement":"Garlic alliinase is a PLP-dependent plant enzyme; its structural cofactor requirement is not a human B6 supplementation experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"ed8c228a-049a-5b0a-a644-863d068bd7ce","mechanism_event_label":"Garlic alliinase is a PLP-dependent plant enzyme; its structural cofactor requirement is not a human B6 supplementation experiment.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"30ba5e07-d4f1-5e89-8aa7-084f7ab9d7a7","slug":"garlic-alliinase","display_name":"Allium sativum alliin lyase / alliinase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ed8c228a-049a-5b0a-a644-863d068bd7ce","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:plp-alliinase-event","event_type":"biochemical_relationship","label":"Garlic alliinase is a PLP-dependent plant enzyme; its structural cofactor requirement is not a human B6 supplementation experiment.","description":"**Alliinase and vitamin B6.** Garlic alliinase is a plant pyridoxal-5′-phosphate (PLP)-dependent enzyme, not a human enzyme. Structural studies resolved its cofactor-containing active site, an apo form, and a PLP-bound aminoacrylate intermediate. Alliin cleavage produces allylsulfenic acid and an aminoacrylate intermediate; the latter yields pyruvate and ammonia. Two allylsulfenic acid molecules condense to allicin with water loss. This links allicin formation to B6 chemistry in the plant. It does not show that taking B6 increases allicin production in a person. [Kuettner 2002](https://pubmed.ncbi.nlm.nih.gov/12235163/) [Shimon 2007](https://pubmed.ncbi.nlm.nih.gov/17174334/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"30ba5e07-d4f1-5e89-8aa7-084f7ab9d7a7","slug":"garlic-alliinase","display_name":"Allium sativum alliin lyase / alliinase","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"663e293b-bb5a-5087-a547-049a1c82ab2f","slug":"alliin","display_name":"Alliin / (+S)-S-allyl-L-cysteine sulfoxide","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified Allium sativum enzyme; X-ray structural study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Garlic alliinase is a PLP-dependent plant enzyme; its structural cofactor requirement is not a human B6 supplementation experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The active principle of garlic at atomic resolution. | 2002 | DOI 10.1074/jbc.m208669200 | PMID 12235163 | https://pubmed.ncbi.nlm.nih.gov/12235163/ | https://doi.org/10.1074/jbc.m208669200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 11-11; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ab10cbd3-b359-5dde-bf65-e88542d7eb7e","evidence_kind":"source_excerpt","locator":"Lines 11-11","start_line":11,"end_line":11,"excerpt":"**Alliinase and vitamin B6.** Garlic alliinase is a plant pyridoxal-5′-phosphate (PLP)-dependent enzyme, not a human enzyme. Structural studies resolved its cofactor-containing active site, an apo form, and a PLP-bound aminoacrylate intermediate. Alliin cleavage produces allylsulfenic acid and an aminoacrylate intermediate; the latter yields pyruvate and ammonia. Two allylsulfenic acid molecules condense to allicin with water loss. This links allicin formation to B6 chemistry in the plant. It does not show that taking B6 increases allicin production in a person. [Kuettner 2002](https://pubmed.ncbi.nlm.nih.gov/12235163/) [Shimon 2007](https://pubmed.ncbi.nlm.nih.gov/17174334/)","model_system":"Purified Allium sativum enzyme; X-ray structural study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}