{"id":"7d25f846-2489-5b09-8ec7-646767ed4ba1","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-gut-local-synthesis","predicate":"increases","statement":"Increasing luminal threonine across 0, 21 and 56 mg/g total amino acids raised measured mucin synthesis in perfused intestinal loops.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6e072de7-bb9f-5336-9b77-1aa6a0605f87","mechanism_event_label":"The local amino-acid supply can change how quickly mucus protein is made.","subject":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"object":{"id":"9c25264f-bc41-5c3b-a05a-fdbf2977bd7f","slug":"pig-luminal-threonine-mucin-synthesis","display_name":"Pig gut-loop mucin synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6e072de7-bb9f-5336-9b77-1aa6a0605f87","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-gut-local-synthesis-event","event_type":"observed_relationship","label":"The local amino-acid supply can change how quickly mucus protein is made.","description":"Increasing luminal threonine across 0, 21 and 56 mg/g total amino acids raised measured mucin synthesis in perfused intestinal loops.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9c25264f-bc41-5c3b-a05a-fdbf2977bd7f","slug":"pig-luminal-threonine-mucin-synthesis","display_name":"Pig gut-loop mucin synthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Six pigs; isolated loops perfused for 120 minutes with an amino-acid mixture and labeled phenylalanine incorporation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An acute local dose response is distinct from chronic whole-diet excess.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The local amino-acid supply can change how quickly mucus protein is made.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luminal threonine concentration acutely affects intestinal mucosal protein and mucin synthesis in piglets. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18567751/ · DOI 10.1093/jn/138.7.1298","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4a869264-1815-55b8-9c55-f7b7b5325ac5","evidence_kind":"source_excerpt","locator":"Lines 210-216","start_line":210,"end_line":216,"excerpt":"## l-threonine-gut-local-synthesis\nThe local amino-acid supply can change how quickly mucus protein is made.\nIncreasing luminal threonine across 0, 21 and 56 mg/g total amino acids raised measured mucin synthesis in perfused intestinal loops.\nModel: Six pigs; isolated loops perfused for 120 minutes with an amino-acid mixture and labeled phenylalanine incorporation.\nLimitations: An acute local dose response is distinct from chronic whole-diet excess.\nEvidence access: Primary abstract\nLuminal threonine concentration acutely affects intestinal mucosal protein and mucin synthesis in piglets. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18567751/ · DOI 10.1093/jn/138.7.1298","model_system":"Six pigs; isolated loops perfused for 120 minutes with an amino-acid mixture and labeled phenylalanine incorporation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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