{"id":"7d05a376-158e-527e-bfe7-192f4a316883","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-selk-message-intact","predicate":"loss_did_not_reduce","statement":"SELENOK deficiency did not impair receptor-induced IP3 production in the reported experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"47326355-1b2c-5240-a43b-d7608f75e4b5","mechanism_event_label":"Failure downstream of IP3 does not automatically mean too little inositol or too little IP3.","subject":{"id":"83ea17c8-4611-596b-9be8-1a0df1c9b42e","slug":"selenok","display_name":"SELENOK","entity_type_key":"protein"},"object":{"id":"3a2e9c8d-faf8-5c95-b1c0-c06852d9ea89","slug":"ip3-production","display_name":"Receptor-evoked IP3 production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"47326355-1b2c-5240-a43b-d7608f75e4b5","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-selk-message-intact-event","event_type":"biochemical_relationship","label":"Failure downstream of IP3 does not automatically mean too little inositol or too little IP3.","description":"SELENOK deficiency did not impair receptor-induced IP3 production in the reported experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83ea17c8-4611-596b-9be8-1a0df1c9b42e","slug":"selenok","display_name":"SELENOK","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3a2e9c8d-faf8-5c95-b1c0-c06852d9ea89","slug":"ip3-production","display_name":"Receptor-evoked IP3 production","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/25368151.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\", \"start_char\": 0, \"end_char\": 1873, \"text_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Immune-cell perturbation, co-immunoprecipitation and palmitoylation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Selk deletion, low-selenium media, DHHC6 knockdown and IP3R cysteine mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selenium limitation and genetic deletion are distinct. This does not establish that inositol supplements repair selenium-dependent receptor failure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mouse immune cells and mammalian cell models","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Failure downstream of IP3 does not automatically mean too little inositol or too little IP3.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. 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This does not establish that inositol supplements repair selenium-dependent receptor failure.\nexposure: Selk deletion, low-selenium media, DHHC6 knockdown and IP3R cysteine mutants\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/25368151.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\", \"start_char\": 0, \"end_char\": 1873, \"text_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\"}\n[ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. 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