{"id":"7cfc9198-7f3c-5c8e-9c90-1e2fd5fcaab6","stable_key":"research:glud1-ampylation-decreases-activity","predicate":"has-reduced-activity-relative-to","statement":"AMPylation decreased GLUD1 enzymatic activity in the tested assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"negative","is_public":true,"mechanism_event_id":"deae10cd-f408-5b28-8aa9-fbd0e959055e","mechanism_event_label":"The AMP modification reduced GLUD1 activity in these experiments.","subject":{"id":"017b8a87-e258-5741-a0ec-7041d32c5c8f","slug":"ampylated-glud1","display_name":"AMPylated GLUD1","entity_type_key":"protein_state"},"object":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"deae10cd-f408-5b28-8aa9-fbd0e959055e","stable_key":"research:glud1-ampylation-decreases-activity","event_type":"enzyme-regulation","label":"The AMP modification reduced GLUD1 activity in these experiments.","description":"AMPylation decreased GLUD1 enzymatic activity in the tested assays.","status":"active","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"017b8a87-e258-5741-a0ec-7041d32c5c8f","slug":"ampylated-glud1","display_name":"AMPylated GLUD1","entity_type_key":"protein_state"},"role":"modified-enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"role":"comparison","stoichiometry":null,"state_label":"unmodified","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"experimental assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Modified-enzyme activity measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No human dietary response established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mammalian","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e0902b8a-5b44-5fa5-bfc3-638b0caa3fb9","evidence_kind":"curated_literature_summary","locator":"lines 498-508","start_line":498,"end_line":508,"excerpt":"## glud1-ampylation-decreases-activity\n\nThe AMP modification reduced GLUD1 activity in these experiments.\n\nAMPylation decreased GLUD1 enzymatic activity in the tested assays.\n\nOrganism: mammalian\nCell type: experimental assay\nExperimental model: Modified-enzyme activity measurements\nLimitations: No human dietary response established.\nPrimary reference: [A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism](https://www.nature.com/articles/s41467-025-63014-z)","model_system":"Modified-enzyme activity measurements","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"mitochondrial-metabolism","plain_language":"The AMP modification reduced GLUD1 activity in these experiments.","evidence_scope":"direct_experimental","papers":[{"key":"gonzalez-2025-mitochondrial-ampylation","title":"A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism","url":"https://www.nature.com/articles/s41467-025-63014-z","doi":"10.1038/s41467-025-63014-z","year":2025,"model":"Biochemical assays and mammalian cells","summary":"Maps mitochondrial AMPylation substrates and tests inhibition of GLUD1 activity."}]}}