{"id":"7cb7b990-22c7-5910-be6c-c4e23376e11c","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-urinary-metabolites","predicate":"is_excreted_with_metabolites","statement":"Biotin represented more than half of recovered urinary biotin-related material; bisnorbiotin, sulfoxide and other metabolites contributed the remainder.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"86ef048c-7244-5ae0-92d2-4bf12207505d","mechanism_event_label":"A urine assay must distinguish intact biotin from its breakdown products.","subject":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"object":{"id":"0e1d7a8a-4638-54eb-b343-1aaec3d23699","slug":"human-urinary-biotin-metabolites","display_name":"Human urinary biotin-metabolite excretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"86ef048c-7244-5ae0-92d2-4bf12207505d","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-urinary-metabolites-event","event_type":"observed_intervention","label":"A urine assay must distinguish intact biotin from its breakdown products.","description":"Biotin represented more than half of recovered urinary biotin-related material; bisnorbiotin, sulfoxide and other metabolites contributed the remainder.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8e9378f0-3a73-5891-8afd-22825626c94c","slug":"bisnorbiotin","display_name":"Bisnorbiotin","entity_type_key":"small_molecule"},"role":"catabolite","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"185f55b3-4892-5c11-9b49-e4c149f249fd","slug":"biotin-sulfoxide","display_name":"Biotin sulfoxide","entity_type_key":"small_molecule"},"role":"catabolite","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0e1d7a8a-4638-54eb-b343-1aaec3d23699","slug":"human-urinary-biotin-metabolites","display_name":"Human urinary biotin-metabolite excretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/10075337.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8eb8ff3bb786de29bfda6f596af2fe6e8fdf366d9cad84065176a825d6035ebd\", \"start_char\": 0, \"end_char\": 1677, \"text_sha256\": \"8eb8ff3bb786de29bfda6f596af2fe6e8fdf366d9cad84065176a825d6035ebd\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crossover oral and intravenous administration in 6 healthy adults","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Oral 2.1, 8.2 or 81.9 micromol; intravenous 18.4 micromol biotin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Bioavailability was inferred from urinary recovery; the anomalous smallest dose and small healthy sample limit generalization to malabsorption or transporter disease.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A urine assay must distinguish intact biotin from its breakdown products.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p10075337] Bioavailability of biotin given orally to humans in pharmacologic doses. (1999). https://pubmed.ncbi.nlm.nih.gov/10075337/ DOI: 10.1093/ajcn/69.3.504","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Whole-body urinary recovery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dcf4c1e8-7cad-549c-94be-89aa225020fb","evidence_kind":"source_excerpt","locator":"Lines 338-349","start_line":338,"end_line":349,"excerpt":"### b7-urinary-metabolites\nBiotin represented more than half of recovered urinary biotin-related material; bisnorbiotin, sulfoxide and other metabolites contributed the remainder.\nCondition category: normal\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A urine assay must distinguish intact biotin from its breakdown products.\norganism: Homo sapiens\ntissue_or_cell_type: Whole-body urinary recovery\nexperimental_model: Crossover oral and intravenous administration in 6 healthy adults\nlimitations: Bioavailability was inferred from urinary recovery; the anomalous smallest dose and small healthy sample limit generalization to malabsorption or transporter disease.\nexposure: Oral 2.1, 8.2 or 81.9 micromol; intravenous 18.4 micromol biotin\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/10075337.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8eb8ff3bb786de29bfda6f596af2fe6e8fdf366d9cad84065176a825d6035ebd\", \"start_char\": 0, \"end_char\": 1677, \"text_sha256\": \"8eb8ff3bb786de29bfda6f596af2fe6e8fdf366d9cad84065176a825d6035ebd\"}\n[b7-p10075337] Bioavailability of biotin given orally to humans in pharmacologic doses. 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