{"id":"7c78fb88-3f54-510b-a2f6-645b31f7752e","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-carnmt1-new-target","predicate":"methylates","statement":"Substrate profiling identified RNF113A as a CARNMT1 histidine-methylation target.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c1adaa1d-0c5e-5f43-8ab7-7fce746302d7","mechanism_event_label":"A shared enzyme creates another testable connection.","subject":{"id":"02c057af-ec05-545c-8bcb-73ef7b9b3401","slug":"human-carnmt1","display_name":"Human carnosine N-methyltransferase / CARNMT1","entity_type_key":"protein"},"object":{"id":"3619ba03-b1a5-5e6c-bc16-70be948034f1","slug":"human-rnf113a","display_name":"Human RNF113A","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c1adaa1d-0c5e-5f43-8ab7-7fce746302d7","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-carnmt1-new-target-event","event_type":"observed_relationship","label":"A shared enzyme creates another testable connection.","description":"Substrate profiling identified RNF113A as a CARNMT1 histidine-methylation target.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"02c057af-ec05-545c-8bcb-73ef7b9b3401","slug":"human-carnmt1","display_name":"Human carnosine N-methyltransferase / CARNMT1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3619ba03-b1a5-5e6c-bc16-70be948034f1","slug":"human-rnf113a","display_name":"Human RNF113A","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7a3c6c7a-6ee3-5f78-9162-7aa2ea1f9bb5","slug":"human-methylated-rnf113a","display_name":"Human histidine-methylated RNF113A","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Peptide-array and cellular methylation profiling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Zinc-finger identity does not establish regulation by zinc intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A shared enzyme creates another testable connection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification of substrates and sequence requirements for CARNMT1-mediated histidine methylation of C3H zinc fingers. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40473212/ · DOI 10.1016/j.jbc.2025.110335","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6053f01d-5226-5ffb-b493-95962fabc6bc","evidence_kind":"source_excerpt","locator":"Lines 164-170","start_line":164,"end_line":170,"excerpt":"## carnosine-carnmt1-new-target\nA shared enzyme creates another testable connection.\nSubstrate profiling identified RNF113A as a CARNMT1 histidine-methylation target.\nModel: Peptide-array and cellular methylation profiling.\nLimitations: Zinc-finger identity does not establish regulation by zinc intake.\nEvidence access: Primary abstract\nIdentification of substrates and sequence requirements for CARNMT1-mediated histidine methylation of C3H zinc fingers. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40473212/ · DOI 10.1016/j.jbc.2025.110335","model_system":"Peptide-array and cellular methylation profiling.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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