{"id":"7c72b746-1a62-5ea1-ad6b-ee00b61ea581","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-galactan-site-null","predicate":"did_not_inhibit","statement":"Synthetic fragments representing the common pectic galactan configuration did not inhibit the tested galectins in this competition assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ebab6dbd-c2a1-5274-b443-678d9ed0723e","mechanism_event_label":"The expected side-chain motif did not function as a strong blocking agent in this assay.","subject":{"id":"fe63daec-e68f-5d3f-89da-f004e2165a98","slug":"pectic-galactan","display_name":"Pectic galactan side chains","entity_type_key":"chemical_species"},"object":{"id":"2289dc7b-77f6-54aa-b3ad-3384ce56df63","slug":"human-gal3-canonical-binding","display_name":"Human galectin-3 canonical carbohydrate-site binding","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ebab6dbd-c2a1-5274-b443-678d9ed0723e","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-galactan-site-null-event","event_type":"biochemical_relationship","label":"The expected side-chain motif did not function as a strong blocking agent in this assay.","description":"Synthetic fragments representing the common pectic galactan configuration did not inhibit the tested galectins in this competition assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fe63daec-e68f-5d3f-89da-f004e2165a98","slug":"pectic-galactan","display_name":"Pectic galactan side chains","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2289dc7b-77f6-54aa-b3ad-3384ce56df63","slug":"human-gal3-canonical-binding","display_name":"Human galectin-3 canonical carbohydrate-site binding","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/pectin-research/27129206.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\", \"start_char\": 0, \"end_char\": 1776, \"text_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Fluorescence-anisotropy competition and cell assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Panel of bioactive pectins including modified products; synthetic pectic fragments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preparation- and assay-specific. This challenges a general canonical-site blockade explanation; it does not disprove all physical interactions or every proposed noncanonical mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pectin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pectin","display_name":"Pectin, structurally heterogeneous plant polysaccharides","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Recombinant galectins and cultured cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The expected side-chain motif did not function as a strong blocking agent in this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[pectin-p27129206] Low or No Inhibitory Potency of the Canonical Galectin Carbohydrate-binding Site by Pectins and Galactomannans. (2016). https://pubmed.ncbi.nlm.nih.gov/27129206/ DOI: 10.1074/jbc.m116.721464","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Canonical carbohydrate-binding site","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"aaffea1c-08eb-5a7a-a62a-be1f25a07b76","evidence_kind":"source_excerpt","locator":"Lines 477-488","start_line":477,"end_line":488,"excerpt":"### pectin-galactan-site-null\nSynthetic fragments representing the common pectic galactan configuration did not inhibit the tested galectins in this competition assay.\nCondition category: normal\nnutrient_topic: Pectin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The expected side-chain motif did not function as a strong blocking agent in this assay.\norganism: Recombinant galectins and cultured cells\ntissue_or_cell_type: Canonical carbohydrate-binding site\nexperimental_model: Fluorescence-anisotropy competition and cell assays\nlimitations: Preparation- and assay-specific. This challenges a general canonical-site blockade explanation; it does not disprove all physical interactions or every proposed noncanonical mechanism.\nexposure: Panel of bioactive pectins including modified products; synthetic pectic fragments\nevidence_span: {\"source_cache\": \"artifacts/pectin-research/27129206.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\", \"start_char\": 0, \"end_char\": 1776, \"text_sha256\": \"b9faed8d7344a109bfebf8e340281cc15375deb88a653cd129967a7084e9eb12\"}\n[pectin-p27129206] Low or No Inhibitory Potency of the Canonical Galectin Carbohydrate-binding Site by Pectins and Galactomannans. (2016). https://pubmed.ncbi.nlm.nih.gov/27129206/ DOI: 10.1074/jbc.m116.721464","model_system":"Fluorescence-anisotropy competition and cell assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [pectin-p27129206] Low or No Inhibitory Potency of the Canonical Galectin Carbohydrate-binding Site by Pectins and Galactomannans. (2016). https://pubmed.ncbi.nlm.nih.gov/27129206/ DOI: 10.1074/jbc.m116.721464","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"13c3aae7-702f-5bc9-9fca-a57d141882ce","stable_key":"import-d17caac5-7018-5ca8-9e96-95afeb384bc4","title":"Pectin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ff07001bc37d008a1e23fbba37146d0b0d33cc8c0bf42a75f1e1a7ef0aaeb489","revision_id":"76f1a2c5-02d0-511b-bde5-33079d7af6a0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}