{"id":"7c5c5a96-4ebe-557d-9732-bc8b3df35889","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-widr-repletion-fails","predicate":"repletion_does_not_restore","statement":"Added tryptophan did not reverse IFN-gamma growth suppression in WiDr cells despite induced tryptophan catabolism.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"1c7fe153-1839-59bd-b98d-63f5bd1f0857","mechanism_event_label":"Another cell type retained suppression after the nutrient was replaced.","subject":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"object":{"id":"8997528a-5194-5c62-b12a-b503a8a799bc","slug":"human-widr-proliferation","display_name":"Proliferation of human WiDr cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1c7fe153-1839-59bd-b98d-63f5bd1f0857","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-widr-repletion-fails-event","event_type":"observed_relationship","label":"Another cell type retained suppression after the nutrient was replaced.","description":"Added tryptophan did not reverse IFN-gamma growth suppression in WiDr cells despite induced tryptophan catabolism.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8997528a-5194-5c62-b12a-b503a8a799bc","slug":"human-widr-proliferation","display_name":"Proliferation of human WiDr cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"393e7fb8-8b09-5b6a-b7e4-a31a78b4fcfb","slug":"ifng","display_name":"Human interferon gamma / IFNG","entity_type_key":"cytokine"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9edba4aa-c2f5-5a4e-9750-15e9a38b1822","slug":"ido1","display_name":"Human indoleamine 2,3-dioxygenase 1 / IDO1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human WiDr culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This experimentally observed context difference is not a contradiction requiring a universal single explanation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Another cell type retained suppression after the nutrient was replaced.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Induction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gamma. · 1988 · https://pubmed.ncbi.nlm.nih.gov/3124115/ · DOI 10.1073/pnas.85.4.1242","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2c59f416-3f5f-5313-9d6b-33e73092cd0a","evidence_kind":"source_excerpt","locator":"Lines 338-344","start_line":338,"end_line":344,"excerpt":"## tryptophan-widr-repletion-fails\nAnother cell type retained suppression after the nutrient was replaced.\nAdded tryptophan did not reverse IFN-gamma growth suppression in WiDr cells despite induced tryptophan catabolism.\nModel: Human WiDr culture.\nLimitations: This experimentally observed context difference is not a contradiction requiring a universal single explanation.\nEvidence access: Primary abstract\nInduction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gamma. · 1988 · https://pubmed.ncbi.nlm.nih.gov/3124115/ · DOI 10.1073/pnas.85.4.1242","model_system":"Human WiDr culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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