{"id":"7c457ba7-ba5f-5c2a-91f7-e05480beb752","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-coalation-reversal","predicate":"reversibly_loses_coa","statement":"After removal of hydrogen peroxide from HEK293/Pank1beta cultures, induced protein CoAlation declined within 5 minutes and returned to baseline within 90 minutes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c64dae9c-5149-5f57-adc4-069cabc1167d","mechanism_event_label":"The CoA modification receded when the oxidative exposure ended.","subject":{"id":"1f79fec8-f7a0-5537-a03a-e74fb90b6c0b","slug":"protein-cysteine-coa-disulfide","display_name":"Protein cysteine–CoA mixed disulfides","entity_type_key":"protein_state"},"object":{"id":"9cb713ef-3378-5882-934c-bd037e5edadc","slug":"protein-coalation","display_name":"Protein cysteine CoAlation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c64dae9c-5149-5f57-adc4-069cabc1167d","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-coalation-reversal-event","event_type":"biochemical_relationship","label":"The CoA modification receded when the oxidative exposure ended.","description":"After removal of hydrogen peroxide from HEK293/Pank1beta cultures, induced protein CoAlation declined within 5 minutes and returned to baseline within 90 minutes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"reversible_modifier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"removed_trigger","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1f79fec8-f7a0-5537-a03a-e74fb90b6c0b","slug":"protein-cysteine-coa-disulfide","display_name":"Protein cysteine–CoA mixed disulfides","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9cb713ef-3378-5882-934c-bd037e5edadc","slug":"protein-coalation","display_name":"Protein cysteine CoAlation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text line 115; Fig. 2C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Oxidant washout in HEK293 cells stably overexpressing Pank1beta","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"500 micromolar H2O2 for 60 minutes, then recovery in complete growth medium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The removal mechanism was not identified; engineered high-CoA cells are not ordinary circulating blood cells. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The CoA modification receded when the oxidative exposure ended.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. (2017). https://pubmed.ncbi.nlm.nih.gov/28341808/ DOI: 10.1042/bcj20170129","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Engineered HEK293 cultures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e7b1417c-4556-516a-92f0-7945c77fd63c","evidence_kind":"source_excerpt","locator":"Lines 1061-1073","start_line":1061,"end_line":1073,"excerpt":"### b5-met-coalation-reversal\nAfter removal of hydrogen peroxide from HEK293/Pank1beta cultures, induced protein CoAlation declined within 5 minutes and returned to baseline within 90 minutes.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The CoA modification receded when the oxidative exposure ended.\norganism: Homo sapiens\ntissue_or_cell_type: Engineered HEK293 cultures\nexperimental_model: Oxidant washout in HEK293 cells stably overexpressing Pank1beta\nlimitations: The removal mechanism was not identified; engineered high-CoA cells are not ordinary circulating blood cells. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: 500 micromolar H2O2 for 60 minutes, then recovery in complete growth medium.\ncross_nutrient: false\nevidence_location: Full text line 115; Fig. 2C\n[b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. (2017). https://pubmed.ncbi.nlm.nih.gov/28341808/ DOI: 10.1042/bcj20170129","model_system":"Oxidant washout in HEK293 cells stably overexpressing Pank1beta","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. 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