{"id":"7c2df639-bd1c-5cea-b577-7a389ad1ac6c","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-surface-pendrin-cftr","predicate":"enhances","statement":"Inducing pendrin approximately doubled the forskolin-stimulated CFTR-sensitive current in the tested cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5549c875-9c16-564f-a8a6-9aeac85cd9df","mechanism_event_label":"One transporter can influence another channel’s measured activity.","subject":{"id":"57fef31d-e7e6-5b6f-aee1-77fb94592ce7","slug":"slc26a4","display_name":"Human pendrin / SLC26A4","entity_type_key":"protein"},"object":{"id":"0b58050c-43bb-5880-9851-cc660e64d9c4","slug":"cftr-anion-conductance","display_name":"CFTR anion conductance","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5549c875-9c16-564f-a8a6-9aeac85cd9df","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-surface-pendrin-cftr-event","event_type":"biochemical_relationship","label":"One transporter can influence another channel’s measured activity.","description":"Inducing pendrin approximately doubled the forskolin-stimulated CFTR-sensitive current in the tested cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53064670-c3d1-59e8-aeaa-a8d673d00f3a","slug":"cftr","display_name":"Human cystic fibrosis transmembrane conductance regulator / CFTR","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"57fef31d-e7e6-5b6f-aee1-77fb94592ce7","slug":"slc26a4","display_name":"Human pendrin / SLC26A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0b58050c-43bb-5880-9851-cc660e64d9c4","slug":"cftr-anion-conductance","display_name":"CFTR anion conductance","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/30742493.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1ff038684ceacd46ad1e237d0cf13337924ec0f611d00a373cd3cbeeeb31864a\", \"start_char\": 0, \"end_char\": 1882, \"text_sha256\": \"1ff038684ceacd46ad1e237d0cf13337924ec0f611d00a373cd3cbeeeb31864a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary epithelial cultures and native tissue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"IL-4 induction and pendrin knockdown","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inflamed surface epithelium differs from Calu-3; no universal airway assignment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human nasal and bronchial epithelia","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"One transporter can influence another channel’s measured activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p30742493] Pendrin Mediates Bicarbonate Secretion and Enhances Cystic Fibrosis Transmembrane Conductance Regulator Function in Airway Surface Epithelia. (2019). https://pubmed.ncbi.nlm.nih.gov/30742493/ DOI: 10.1165/rcmb.2018-0158oc","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Ciliated airway surface","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8282b42e-0fe4-5620-8385-0fe0d1c4ebfe","evidence_kind":"source_excerpt","locator":"Lines 1069-1080","start_line":1069,"end_line":1080,"excerpt":"### chloride-surface-pendrin-cftr\nInducing pendrin approximately doubled the forskolin-stimulated CFTR-sensitive current in the tested cells.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: One transporter can influence another channel’s measured activity.\norganism: Human nasal and bronchial epithelia\ntissue_or_cell_type: Ciliated airway surface\nexperimental_model: Primary epithelial cultures and native tissue\nlimitations: Inflamed surface epithelium differs from Calu-3; no universal airway assignment.\nexposure: IL-4 induction and pendrin knockdown\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/30742493.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1ff038684ceacd46ad1e237d0cf13337924ec0f611d00a373cd3cbeeeb31864a\", \"start_char\": 0, \"end_char\": 1882, \"text_sha256\": \"1ff038684ceacd46ad1e237d0cf13337924ec0f611d00a373cd3cbeeeb31864a\"}\n[chloride-p30742493] Pendrin Mediates Bicarbonate Secretion and Enhances Cystic Fibrosis Transmembrane Conductance Regulator Function in Airway Surface Epithelia. 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