{"id":"7b9c69b1-af3a-54c2-8bdb-c6e0d1bbb87b","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-oral-k-aldosterone-independent-ncc-off","predicate":"oral-loading-decreases","statement":"Oral K rapidly dephosphorylated NCC in mice, including aldosterone-deficient animals.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c9bade22-50bc-55bb-8a01-775382fcf844","mechanism_event_label":"The earliest NCC response to potassium does not require a new aldosterone signal in this model.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"8197e69c-215b-5cd4-bf4b-edde6708ea8f","slug":"ncc-phosphorylation","display_name":"NCC phosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c9bade22-50bc-55bb-8a01-775382fcf844","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-oral-k-aldosterone-independent-ncc-off-event","event_type":"biochemical_relationship","label":"The earliest NCC response to potassium does not require a new aldosterone signal in this model.","description":"Oral K rapidly dephosphorylated NCC in mice, including aldosterone-deficient animals.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"causal-subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8197e69c-215b-5cd4-bf4b-edde6708ea8f","slug":"ncc-phosphorylation","display_name":"NCC phosphorylation","entity_type_key":"cellular_process"},"role":"measured-target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4ed6f3e2-76e5-577a-b3bb-64703cf550fa","slug":"aldosterone","display_name":"Aldosterone","entity_type_key":"small_molecule"},"role":"tested-nonrequirement","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"63291643-1df9-5547-952a-9082838a350e","slug":"slc12a3","display_name":"Thiazide-sensitive sodium-chloride cotransporter","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"delivered-ion","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"K loading suppresses a Na/Cl transporter before some hormonal adaptations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract; early NCC time course and aldosterone-deficient mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Gastric K load; aldosterone-deficient comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Early response differs from later ENaC activation; acute gavage is not a chronic diet.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The earliest NCC response to potassium does not require a new aldosterone signal in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sorensen-2013-oral-k-ncc] Rapid dephosphorylation of the renal sodium chloride cotransporter in response to oral potassium intake in mice (2013). https://pubmed.ncbi.nlm.nih.gov/23447069/ DOI: 10.1038/ki.2013.14","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney DCT","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ca301421-df50-517c-9517-81efaee9178d","evidence_kind":"source_excerpt","locator":"Lines 294-305","start_line":294,"end_line":305,"excerpt":"### renal-oral-k-aldosterone-independent-ncc-off\nOral K rapidly dephosphorylated NCC in mice, including aldosterone-deficient animals.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The earliest NCC response to potassium does not require a new aldosterone signal in this model.\norganism: Mus musculus\ntissue_or_cell_type: Kidney DCT\nexperimental_model: Gastric K load; aldosterone-deficient comparison\nlimitations: Early response differs from later ENaC activation; acute gavage is not a chronic diet.\ncross_nutrient: K loading suppresses a Na/Cl transporter before some hormonal adaptations.\nevidence_location: Primary abstract; early NCC time course and aldosterone-deficient mice.\n[sorensen-2013-oral-k-ncc] Rapid dephosphorylation of the renal sodium chloride cotransporter in response to oral potassium intake in mice (2013). https://pubmed.ncbi.nlm.nih.gov/23447069/ DOI: 10.1038/ki.2013.14","model_system":"Gastric K load; aldosterone-deficient comparison","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sorensen-2013-oral-k-ncc] Rapid dephosphorylation of the renal sodium chloride cotransporter in response to oral potassium intake in mice (2013). https://pubmed.ncbi.nlm.nih.gov/23447069/ DOI: 10.1038/ki.2013.14","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}