{"id":"7b6f4d95-4eab-545e-ba57-b84aab0fe5ca","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-enz-ca6-inhibition","predicate":"inhibits","statement":"Acetazolamide inhibited salivary and milk human CA6 with reported Ki values of 16 and 23 nM in the enzyme assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"67ddaa59-2b3a-548c-8e0a-22e0183dce76","mechanism_event_label":"A carbonic anhydrase inhibitor blocked the purified secreted enzyme.","subject":{"id":"fb687c28-449a-544b-a5e4-d584c1021271","slug":"acetazolamide","display_name":"Acetazolamide","entity_type_key":"small_molecule"},"object":{"id":"1702b00c-5ca3-56bb-9136-503543f60dae","slug":"ca6","display_name":"Human carbonic anhydrase VI / CA6","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"67ddaa59-2b3a-548c-8e0a-22e0183dce76","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-enz-ca6-inhibition-event","event_type":"biochemical_relationship","label":"A carbonic anhydrase inhibitor blocked the purified secreted enzyme.","description":"Acetazolamide inhibited salivary and milk human CA6 with reported Ki values of 16 and 23 nM in the enzyme assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"enzyme_cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fb687c28-449a-544b-a5e4-d584c1021271","slug":"acetazolamide","display_name":"Acetazolamide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1702b00c-5ca3-56bb-9136-503543f60dae","slug":"ca6","display_name":"Human carbonic anhydrase VI / CA6","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CA6 purified from pooled saliva or breast milk; stopped-flow enzymology","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Inhibitor dilution series in stopped-flow enzymology; not oral treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay inhibition does not measure nutrient deficiency or treatment efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A carbonic anhydrase inhibitor blocked the purified secreted enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-enz-ca6-2022] Biochemical and Biophysical Characterization of Carbonic Anhydrase VI from Human Milk and Saliva. (2022). https://pubmed.ncbi.nlm.nih.gov/35947329/ DOI: 10.1007/s10930-022-10070-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified human saliva and milk CA6","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5b0e8b95-0675-564b-b9c6-ecb573c8f473","evidence_kind":"source_excerpt","locator":"Lines 677-688","start_line":677,"end_line":688,"excerpt":"### zinc-enz-ca6-inhibition\nAcetazolamide inhibited salivary and milk human CA6 with reported Ki values of 16 and 23 nM in the enzyme assay.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A carbonic anhydrase inhibitor blocked the purified secreted enzyme.\norganism: Homo sapiens\ntissue_or_cell_type: Purified human saliva and milk CA6\nexperimental_model: Human CA6 purified from pooled saliva or breast milk; stopped-flow enzymology\nlimitations: Assay inhibition does not measure nutrient deficiency or treatment efficacy.\nexposure: Inhibitor dilution series in stopped-flow enzymology; not oral treatment.\ncross_nutrient: false\n[zinc-enz-ca6-2022] Biochemical and Biophysical Characterization of Carbonic Anhydrase VI from Human Milk and Saliva. (2022). https://pubmed.ncbi.nlm.nih.gov/35947329/ DOI: 10.1007/s10930-022-10070-9","model_system":"Human CA6 purified from pooled saliva or breast milk; stopped-flow enzymology","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-enz-ca6-2022] Biochemical and Biophysical Characterization of Carbonic Anhydrase VI from Human Milk and Saliva. (2022). https://pubmed.ncbi.nlm.nih.gov/35947329/ DOI: 10.1007/s10930-022-10070-9","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}