{"id":"7b675056-7336-5ed3-a133-fcd559ef6d1e","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-sodium-coupling","predicate":"catalyzes","statement":"Rat NIS expressed in Xenopus oocytes transported iodide with two sodium ions per anion and generated inward electrogenic transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"915c3bd9-6710-5e14-b701-1f5acf0f6010","mechanism_event_label":"NIS uses sodium movement to carry iodide into cells.","subject":{"id":"acfbc5ec-b299-5365-8c5d-9e492c18d1d9","slug":"rat-slc5a5","display_name":"Rat sodium/iodide symporter / Slc5a5","entity_type_key":"protein"},"object":{"id":"7bb54a03-32ad-5b7a-8666-2858d4f95038","slug":"nis-sodium-iodide-cotransport","display_name":"NIS sodium-iodide cotransport","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"915c3bd9-6710-5e14-b701-1f5acf0f6010","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-sodium-coupling-event","event_type":"biochemical_relationship","label":"NIS uses sodium movement to carry iodide into cells.","description":"Rat NIS expressed in Xenopus oocytes transported iodide with two sodium ions per anion and generated inward electrogenic transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"cotransported_ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0e4aaa90-5b94-5d69-ba79-d760da4e0268","slug":"sodium","display_name":"Sodium","entity_type_key":"nutrient_element"},"role":"interacting_nutrient","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"acfbc5ec-b299-5365-8c5d-9e492c18d1d9","slug":"rat-slc5a5","display_name":"Rat sodium/iodide symporter / Slc5a5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7bb54a03-32ad-5b7a-8666-2858d4f95038","slug":"nis-sodium-iodide-cotransport","display_name":"NIS sodium-iodide cotransport","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat NIS expressed in Xenopus laevis oocytes; electrophysiology and tracer uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Tracer uptake and electrophysiology; apparent sodium affinity 28 ± 3 mM and iodide affinity 33 ± 9 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rat-protein assay parameters are not dietary sodium requirements or human iodide thresholds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat protein in Xenopus laevis oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"NIS uses sodium movement to carry iodide into cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-stoichiometry1997] Thyroid Na+/I- symporter. Mechanism, stoichiometry, and specificity. (1997). https://pubmed.ncbi.nlm.nih.gov/9341168/ DOI: 10.1074/jbc.272.43.27230","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"665664a9-3738-53e9-8582-5b5fb9d123e8","evidence_kind":"source_excerpt","locator":"Lines 167-178","start_line":167,"end_line":178,"excerpt":"### iodine-trans-sodium-coupling\nRat NIS expressed in Xenopus oocytes transported iodide with two sodium ions per anion and generated inward electrogenic transport.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: NIS uses sodium movement to carry iodide into cells.\norganism: Rat protein in Xenopus laevis oocytes\ntissue_or_cell_type: Oocyte plasma membrane\nexperimental_model: Rat NIS expressed in Xenopus laevis oocytes; electrophysiology and tracer uptake\nlimitations: Rat-protein assay parameters are not dietary sodium requirements or human iodide thresholds.\nexposure: Tracer uptake and electrophysiology; apparent sodium affinity 28 ± 3 mM and iodide affinity 33 ± 9 micromolar.\ncross_nutrient: true\n[iodine-trans-stoichiometry1997] Thyroid Na+/I- symporter. Mechanism, stoichiometry, and specificity. (1997). https://pubmed.ncbi.nlm.nih.gov/9341168/ DOI: 10.1074/jbc.272.43.27230","model_system":"Rat NIS expressed in Xenopus laevis oocytes; electrophysiology and tracer uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-trans-stoichiometry1997] Thyroid Na+/I- symporter. Mechanism, stoichiometry, and specificity. (1997). https://pubmed.ncbi.nlm.nih.gov/9341168/ DOI: 10.1074/jbc.272.43.27230","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a4885eb6f58bad4a4514c958b63834619e57b10aa7f694aa97d0f65c7d36548f","revision_id":"ea8001a1-b576-5eda-a193-883c7ad59b73","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}