{"id":"7b2b2ed0-5d87-53aa-84c2-405554cb1321","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-nac-interaction","predicate":"interacts_in_cell_free_system_with","statement":"UV spectroscopy and FTICR mass spectrometry detected physical interaction between cucurbitacin B and N-acetylcysteine in a cell-free system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f82d6085-df2d-5021-8550-bc9aa8e6b685","mechanism_event_label":"An antioxidant can also change the amount of available test compound.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f82d6085-df2d-5021-8550-bc9aa8e6b685","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-nac-interaction-event","event_type":"observed_relationship","label":"An antioxidant can also change the amount of available test compound.","description":"UV spectroscopy and FTICR mass spectrometry detected physical interaction between cucurbitacin B and N-acetylcysteine in a cell-free system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"NAC rescue therefore does not uniquely prove ROS scavenging; it also does not rule out a role for ROS.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"An antioxidant can also change the amount of available test compound.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B potently suppresses non-small-cell lung cancer growth: identification of intracellular thiols as critical targets. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23340170/ · DOI 10.1016/j.canlet.2013.01.008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dd766da1-5fe5-575a-9cea-c648cc2063c0","evidence_kind":"source_excerpt","locator":"Lines 116-122","start_line":116,"end_line":122,"excerpt":"## cucurbitacin-b-nac-interaction\nAn antioxidant can also change the amount of available test compound.\nUV spectroscopy and FTICR mass spectrometry detected physical interaction between cucurbitacin B and N-acetylcysteine in a cell-free system.\nModel: Cell-free chemistry.\nLimitations: NAC rescue therefore does not uniquely prove ROS scavenging; it also does not rule out a role for ROS.\nEvidence access: Primary abstract\nCucurbitacin B potently suppresses non-small-cell lung cancer growth: identification of intracellular thiols as critical targets. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23340170/ · DOI 10.1016/j.canlet.2013.01.008","model_system":"Cell-free chemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}