{"id":"7b107029-c5a1-5e92-a27d-2ffa7c59c373","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mthfd1l-matrix-face","predicate":"localizes-to","statement":"Human MTHFD1L expressed in CHO cells behaves as a peripheral protein at the matrix face of the inner mitochondrial membrane.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5d09fa40-1d6b-5fe9-8336-5ea295ed203b","mechanism_event_label":"The formate-pathway enzyme sits on the mitochondrial inner surface.","subject":{"id":"e6e61185-a9fc-5028-92e7-7f7eaf76632f","slug":"mthfd1l","display_name":"Human MTHFD1L","entity_type_key":"protein"},"object":{"id":"4896b3f4-07bf-56d2-a369-c421f8b37ace","slug":"inner-mitochondrial-membrane","display_name":"Inner mitochondrial membrane","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"5d09fa40-1d6b-5fe9-8336-5ea295ed203b","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mthfd1l-matrix-face-event","event_type":"observed_intervention","label":"The formate-pathway enzyme sits on the mitochondrial inner surface.","description":"Human MTHFD1L expressed in CHO cells behaves as a peripheral protein at the matrix face of the inner mitochondrial membrane.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"1f2cf4a9-0168-5691-8b00-1676e5a477e1","slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix","entity_type_key":"organelle"},"role":"membrane-facing compartment","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6e61185-a9fc-5028-92e7-7f7eaf76632f","slug":"mthfd1l","display_name":"Human MTHFD1L","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4896b3f4-07bf-56d2-a369-c421f8b37ace","slug":"inner-mitochondrial-membrane","display_name":"Inner mitochondrial membrane","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Tagged human protein, mitochondrial fractionation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tagging and heterologous expression may affect localization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens protein in Cricetulus griseus cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The formate-pathway enzyme sits on the mitochondrial inner surface.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[prasannan-2009] Human mitochondrial C1-tetrahydrofolate synthase: submitochondrial localization of the full-length enzyme and characterization of a short isoform (2009). https://pubmed.ncbi.nlm.nih.gov/18996079/ DOI: 10.1016/j.abb.2008.10.028","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"CHO cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1230624b-8f16-54cd-83fc-d725b95b22f3","evidence_kind":"source_excerpt","locator":"Lines 875-885","start_line":875,"end_line":885,"excerpt":"### mthfd1l-matrix-face\nHuman MTHFD1L expressed in CHO cells behaves as a peripheral protein at the matrix face of the inner mitochondrial membrane.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The formate-pathway enzyme sits on the mitochondrial inner surface.\norganism: Homo sapiens protein in Cricetulus griseus cells\ntissue_or_cell_type: CHO cells\nexperimental_model: Tagged human protein, mitochondrial fractionation\nlimitations: Tagging and heterologous expression may affect localization.\nexposure: Assay conditions described in the linked primary study.\n[prasannan-2009] Human mitochondrial C1-tetrahydrofolate synthase: submitochondrial localization of the full-length enzyme and characterization of a short isoform (2009). https://pubmed.ncbi.nlm.nih.gov/18996079/ DOI: 10.1016/j.abb.2008.10.028","model_system":"Tagged human protein, mitochondrial fractionation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [prasannan-2009] Human mitochondrial C1-tetrahydrofolate synthase: submitochondrial localization of the full-length enzyme and characterization of a short isoform (2009). https://pubmed.ncbi.nlm.nih.gov/18996079/ DOI: 10.1016/j.abb.2008.10.028","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}