{"id":"7ad4aab1-2e02-5652-8638-658cd6f71889","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-asns-two-sites","predicate":"couples_glutamine_and_aspartate_chemistry","statement":"The human ASNS structure separates glutamine-processing and ATP-dependent synthetase domains, with an internal path supporting nitrogen transfer toward activated aspartate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0c6004be-002b-5c61-a7bc-226c627a1550","mechanism_event_label":"The enzyme coordinates two reactions rather than attaching free glutamine directly to aspartate.","subject":{"id":"d963b556-e89d-551f-960b-24d695590bda","slug":"asns","display_name":"Human asparagine synthetase / ASNS","entity_type_key":"protein"},"object":{"id":"667f5a9c-66f8-5b52-8b7b-08e4d19a0fda","slug":"human-asns-two-domain-catalysis","display_name":"Coupled glutaminase and synthetase domains of human ASNS","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0c6004be-002b-5c61-a7bc-226c627a1550","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-asns-two-sites-event","event_type":"observed_relationship","label":"The enzyme coordinates two reactions rather than attaching free glutamine directly to aspartate.","description":"The human ASNS structure separates glutamine-processing and ATP-dependent synthetase domains, with an internal path supporting nitrogen transfer toward activated aspartate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d963b556-e89d-551f-960b-24d695590bda","slug":"asns","display_name":"Human asparagine synthetase / ASNS","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"667f5a9c-66f8-5b52-8b7b-08e4d19a0fda","slug":"human-asns-two-domain-catalysis","display_name":"Coupled glutaminase and synthetase domains of human ASNS","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human ASNS 1.85-angstrom structure and comparison with mechanistic data.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structural interpretation is distinguished from measuring every transient chemical intermediate in intact cells. Correction record: The 2019 author correction added omitted author affiliations and funding acknowledgements; no mechanism or data change was stated. PMID 31799439; DOI 10.1038/s42003-019-0690-1. https://www.nature.com/articles/s42003-019-0690-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The enzyme coordinates two reactions rather than attaching free glutamine directly to aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"High-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivity. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31552298/ · DOI 10.1038/s42003-019-0587-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ff2954c1-c12b-5359-91ab-baf9ef1fa5a6","evidence_kind":"source_excerpt","locator":"Lines 194-200","start_line":194,"end_line":200,"excerpt":"## l-aspartate-asns-two-sites\nThe enzyme coordinates two reactions rather than attaching free glutamine directly to aspartate.\nThe human ASNS structure separates glutamine-processing and ATP-dependent synthetase domains, with an internal path supporting nitrogen transfer toward activated aspartate.\nModel: Human ASNS 1.85-angstrom structure and comparison with mechanistic data.\nLimitations: Structural interpretation is distinguished from measuring every transient chemical intermediate in intact cells. Correction record: The 2019 author correction added omitted author affiliations and funding acknowledgements; no mechanism or data change was stated. PMID 31799439; DOI 10.1038/s42003-019-0690-1. https://www.nature.com/articles/s42003-019-0690-1\nEvidence access: Primary full text\nHigh-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivity. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31552298/ · DOI 10.1038/s42003-019-0587-z","model_system":"Human ASNS 1.85-angstrom structure and comparison with mechanistic data.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"67970dcb-34e0-5b0a-8c86-d8c3cc183444","stable_key":"import-e37461ea-ea5d-5e2c-8091-138305f6dd70","title":"L-Aspartate: redox transfer, nitrogen partitioning and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a2791036b8f554be57d0b40892634bb9c900fa03e7e4c9c95ca08428ed52d614","revision_id":"150613bb-a227-5ec9-9f5c-fb13f5da50c8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}