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an amino acid supplement was not the tested intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second enzyme complex converts the attached amino acid to the required one.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Biogenesis of glutaminyl-mt tRNAGln in human mitochondria. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19805282/ · DOI 10.1073/pnas.0907602106","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4bb8483a-66cc-5590-8b94-19160a892ff0","evidence_kind":"source_excerpt","locator":"Lines 378-384","start_line":378,"end_line":384,"excerpt":"## glutamate-gatcab-conversion\nA second enzyme complex converts the attached amino acid to the required one.\nRecombinant human mtGluRS and GatCAB reconstituted formation of glutaminyl-tRNA(Gln) through the glutamylated intermediate in vitro.\nModel: Human recombinant mitochondrial tRNA pathway reconstitution.\nLimitations: Each complex subunit is separately modeled; an amino acid supplement was not the tested intervention.\nEvidence access: Primary abstract\nBiogenesis of glutaminyl-mt tRNAGln in human mitochondria. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19805282/ · DOI 10.1073/pnas.0907602106","model_system":"Human recombinant mitochondrial tRNA pathway reconstitution.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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