{"id":"7a552c2d-c4ab-52f2-9f94-6be6dfad16f9","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-hela-lipid-loss","predicate":"silencing_increases","statement":"SLC22A4-depleted human cells showed increased protein oxidation and lipid peroxidation under oxidative stress.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7b048869-e752-533c-9c45-57a72c238a74","mechanism_event_label":"The consequences extend beyond DNA.","subject":{"id":"1509c925-fc5e-5a36-aa59-96bcc489965a","slug":"slc22a4","display_name":"Human ergothioneine transporter OCTN1 / SLC22A4","entity_type_key":"protein"},"object":{"id":"4fe7e74b-2d2d-54e3-ba82-deb79c47f4b5","slug":"human-cell-lipid-peroxidation","display_name":"Human cultured-cell lipid peroxidation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7b048869-e752-533c-9c45-57a72c238a74","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-hela-lipid-loss-event","event_type":"observed_relationship","label":"The consequences extend beyond DNA.","description":"SLC22A4-depleted human cells showed increased protein oxidation and lipid peroxidation under oxidative stress.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1509c925-fc5e-5a36-aa59-96bcc489965a","slug":"slc22a4","display_name":"Human ergothioneine transporter OCTN1 / SLC22A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4fe7e74b-2d2d-54e3-ba82-deb79c47f4b5","slug":"human-cell-lipid-peroxidation","display_name":"Human cultured-cell lipid peroxidation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cultured cells with transporter knockdown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a human dietary deficiency syndrome; linked outcomes share one study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The consequences extend beyond DNA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The unusual amino acid L-ergothioneine is a physiologic cytoprotectant. · 2010 · https://pubmed.ncbi.nlm.nih.gov/19911007/ · DOI 10.1038/cdd.2009.163","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"de536927-cfb5-5422-8377-656544080a74","evidence_kind":"source_excerpt","locator":"Lines 88-94","start_line":88,"end_line":94,"excerpt":"## ergothioneine-hela-lipid-loss\nThe consequences extend beyond DNA.\nSLC22A4-depleted human cells showed increased protein oxidation and lipid peroxidation under oxidative stress.\nModel: Human cultured cells with transporter knockdown.\nLimitations: Not a human dietary deficiency syndrome; linked outcomes share one study.\nEvidence access: Primary abstract\nThe unusual amino acid L-ergothioneine is a physiologic cytoprotectant. · 2010 · https://pubmed.ncbi.nlm.nih.gov/19911007/ · DOI 10.1038/cdd.2009.163","model_system":"Human cultured cells with transporter knockdown.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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