{"id":"79e8d2b0-cd32-5152-bdbe-d143c95a01a7","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfx01-bb7121-pstat3-null","predicate":"has_contextual_experimental_result","statement":"SFX-01 did not repress STAT3 activity in the reported previously SFX-01-treated, resistant BB7121 sample.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e7d106fe-49e6-5095-97d5-7b672f9293b5","mechanism_event_label":"sfx01-bb7121-pstat3-null","subject":{"id":"80b20bc7-d9e7-5909-9e77-838b0e3c8486","slug":"sfx-01","display_name":"SFX-01, sulforaphane stabilized in an alpha-cyclodextrin complex","entity_type_key":"chemical_species"},"object":{"id":"7f3bce97-3272-5acb-bbc2-fb7830e30278","slug":"human-whole-cell-pstat3-abundance","display_name":"Whole-cell phosphorylated STAT3 abundance in human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e7d106fe-49e6-5095-97d5-7b672f9293b5","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfx01-bb7121-pstat3-null","event_type":"experimental_result","label":"sfx01-bb7121-pstat3-null","description":"SFX-01 did not repress STAT3 activity in the reported previously SFX-01-treated, resistant BB7121 sample.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80b20bc7-d9e7-5909-9e77-838b0e3c8486","slug":"sfx-01","display_name":"SFX-01, sulforaphane stabilized in an alpha-cyclodextrin complex","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7f3bce97-3272-5acb-bbc2-fb7830e30278","slug":"human-whole-cell-pstat3-abundance","display_name":"Whole-cell phosphorylated STAT3 abundance in human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"compound in stabilized formulation","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human metastatic breast cancer patient-derived sample BB7121","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Prior clinical SFX-01 exposure with progression; ex vivo STAT3 assay. Exact panel exposure unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli. A single resistant sample does not determine the mechanism of resistance. Supplementary legend/main-text result reviewed; raw image not quantitatively reanalyzed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Results: SFX-01 targets STAT3 signaling; Supplementary Figure S4c.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41388-020-1335-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"edd6b07a-8735-537c-ba79-e12b1f02ca0c","evidence_kind":"source_excerpt","locator":"Lines 105-111","start_line":105,"end_line":111,"excerpt":"SFX-01 did not repress STAT3 activity in the reported previously SFX-01-treated, resistant BB7121 sample.\nprimary_references: https://doi.org/10.1038/s41388-020-1335-z\nprimary_locator: Results: SFX-01 targets STAT3 signaling; Supplementary Figure S4c.\norganism: Human\nexperimental_model: Human metastatic breast cancer patient-derived sample BB7121\nexposure: Prior clinical SFX-01 exposure with progression; ex vivo STAT3 assay. Exact panel exposure unresolved.\nlimitations: The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli. A single resistant sample does not determine the mechanism of resistance. Supplementary legend/main-text result reviewed; raw image not quantitatively reanalyzed.","model_system":"Human metastatic breast cancer patient-derived sample BB7121","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of the specified primary result; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01fc674f-07c6-58fd-b1e7-877478a4472e","stable_key":"import-708c252b-0aef-5cec-95bf-99d19578214c","title":"APT2-STAT3 cycling and a competing sulforaphane target route","document_type":"imported_text","citation_label":"Selected primary observations: 10.1038/s41586-020-2799-2 and 10.1038/s41388-020-1335-z.","file_path":"","sha256":"b1b245468dac7acc94a2fef690d994124b2bc4fc5f92135173cf5636af391395","revision_id":"d84b8419-69dd-50d9-b587-5627ed414591","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}