{"id":"79bdf159-038d-5b08-9d18-166fc850f7d9","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-sulfate-cyp3a4","predicate":"has_limited_effect","statement":"At 20 micromolar, the sulfate reduced testosterone hydroxylation by less than 10% in the human CYP3A4 assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c9dca2ae-44d8-55e8-9e10-3806238432ba","mechanism_event_label":"A transporter interaction does not imply strong CYP inhibition.","subject":{"id":"b71fca58-3e61-5eed-bfa5-4465fcc9407e","slug":"myricetin-3-prime-o-sulfate","display_name":"Myricetin 3′-O-sulfate","entity_type_key":"small_molecule"},"object":{"id":"54e95ff1-4ffa-5e6f-a4cb-b47e5d2a5bda","slug":"cyp3a4","display_name":"Human cytochrome P450 3A4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c9dca2ae-44d8-55e8-9e10-3806238432ba","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-sulfate-cyp3a4-event","event_type":"observed_relationship","label":"A transporter interaction does not imply strong CYP inhibition.","description":"At 20 micromolar, the sulfate reduced testosterone hydroxylation by less than 10% in the human CYP3A4 assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b71fca58-3e61-5eed-bfa5-4465fcc9407e","slug":"myricetin-3-prime-o-sulfate","display_name":"Myricetin 3′-O-sulfate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"54e95ff1-4ffa-5e6f-a4cb-b47e5d2a5bda","slug":"cyp3a4","display_name":"Human cytochrome P450 3A4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzymes; 5 micromolar substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Restricted substrate, concentration and preparation; earlier CYP2C9 assays gave different potency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A transporter interaction does not imply strong CYP inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Interaction of myricetin, ampelopsin (dihydromyricetin), and their sulfate metabolites with serum albumin, cytochrome P450 (CYP2C9, 2C19, and 3A4) enzymes, and organic anion-transporting polypeptides (OATP1B1 and OATP2B1). · 2024 · https://pubmed.ncbi.nlm.nih.gov/39344282/ · DOI 10.1002/prp2.70021","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"daa62638-0cfd-5d38-b5aa-245dd982bf0c","evidence_kind":"source_excerpt","locator":"Lines 196-202","start_line":196,"end_line":202,"excerpt":"## myricetin-sulfate-cyp3a4\nA transporter interaction does not imply strong CYP inhibition.\nAt 20 micromolar, the sulfate reduced testosterone hydroxylation by less than 10% in the human CYP3A4 assay.\nModel: Recombinant human enzymes; 5 micromolar substrate.\nLimitations: Restricted substrate, concentration and preparation; earlier CYP2C9 assays gave different potency.\nEvidence access: Primary full text\nInteraction of myricetin, ampelopsin (dihydromyricetin), and their sulfate metabolites with serum albumin, cytochrome P450 (CYP2C9, 2C19, and 3A4) enzymes, and organic anion-transporting polypeptides (OATP1B1 and OATP2B1). · 2024 · https://pubmed.ncbi.nlm.nih.gov/39344282/ · DOI 10.1002/prp2.70021","model_system":"Recombinant human enzymes; 5 micromolar substrate.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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