{"id":"79b2a21c-5658-598c-9783-60806d617dde","stable_key":"research:txnrd3-mouse-fertility","predicate":"supports_in_tested_model","statement":"Male Txnrd3-knockout mice showed impaired reproductive performance and reduced fertilization in the reported assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"8ec6bbe1-5827-58ce-8fa7-584813c79fa1","mechanism_event_label":"Removing TXNRD3 impaired fertility in the studied mice.","subject":{"id":"bc046427-cd2b-50e4-a482-b37b08495514","slug":"txnrd3","display_name":"TXNRD3","entity_type_key":"protein"},"object":{"id":"8ddf3af8-d323-5d24-8808-3ddf39e6cf76","slug":"male-fertility","display_name":"Male fertility","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8ec6bbe1-5827-58ce-8fa7-584813c79fa1","stable_key":"research:txnrd3-mouse-fertility","event_type":"experimentally_scoped_interaction","label":"Removing TXNRD3 impaired fertility in the studied mice.","description":"Male Txnrd3-knockout mice showed impaired reproductive performance and reduced fertilization in the reported assays.","status":"active","compartment":null,"participants":[{"entity":{"id":"bc046427-cd2b-50e4-a482-b37b08495514","slug":"txnrd3","display_name":"TXNRD3","entity_type_key":"protein"},"role":"regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8ddf3af8-d323-5d24-8808-3ddf39e6cf76","slug":"male-fertility","display_name":"Male fertility","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This experiment-specific relationship does not establish a human dietary-deficiency threshold or supplementation benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cdaeff70-cdd0-5e56-b5c3-d6d6f3297354","evidence_kind":"curated_literature_summary","locator":"lines 987-996","start_line":987,"end_line":996,"excerpt":"## txnrd3-mouse-fertility\n\nRemoving TXNRD3 impaired fertility in the studied mice.\n\nMale Txnrd3-knockout mice showed impaired reproductive performance and reduced fertilization in the reported assays.\n\nExperimental model: Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.\nOrganism: Mouse\nLimitations: This experiment-specific relationship does not establish a human dietary-deficiency threshold or supplementation benefit.\nPrimary reference: [Selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis](https://pmc.ncbi.nlm.nih.gov/articles/PMC9352919/)","model_system":"Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"Reproduction","plain_language":"Removing TXNRD3 impaired fertility in the studied mice.","evidence_scope":"direct_experimental","papers":[{"key":"catalog-txnrd3-2022","title":"Selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9352919/","doi":null,"year":2022,"model":"Txnrd3-knockout mice, sperm thiol assays and in-vitro fertilization.","summary":"Txnrd3 deletion altered sperm thiol redox state and reproductive performance; the enzyme has thioredoxin/glutathione reductase activity in vitro."}]}}