{"id":"796db1c7-5f29-5acf-832d-63a18f0006f9","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-renal-b0at1","predicate":"supports","statement":"Silencing SLC6A19 reduced citrulline uptake in human HK-2 renal cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d024e15c-cdb5-57ba-8fdb-0b64c1ed31cc","mechanism_event_label":"A neutral-amino-acid transporter contributes to kidney uptake.","subject":{"id":"10307ad9-80a3-56be-a7f5-740c9362297f","slug":"slc6a19","display_name":"Human neutral amino acid transporter B0AT1 / SLC6A19","entity_type_key":"protein"},"object":{"id":"14cc9aa4-cc18-5f17-96f8-aee7de56abea","slug":"renal-citrulline-uptake","display_name":"Apical citrulline uptake in renal proximal tubular cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d024e15c-cdb5-57ba-8fdb-0b64c1ed31cc","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-renal-b0at1-event","event_type":"biochemical_relationship","label":"A neutral-amino-acid transporter contributes to kidney uptake.","description":"Silencing SLC6A19 reduced citrulline uptake in human HK-2 renal cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f53c7bfb-3b91-56fe-bebd-28623cfe13a0","slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"10307ad9-80a3-56be-a7f5-740c9362297f","slug":"slc6a19","display_name":"Human neutral amino acid transporter B0AT1 / SLC6A19","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"14cc9aa4-cc18-5f17-96f8-aee7de56abea","slug":"renal-citrulline-uptake","display_name":"Apical citrulline uptake in renal proximal tubular cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/citrulline-research/19322909.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f466fcced77ee9fe7a97f010112a035332d513382d13e6187d10bc02be152ece\", \"start_char\": 0, \"end_char\": 1531, \"text_sha256\": \"f466fcced77ee9fe7a97f010112a035332d513382d13e6187d10bc02be152ece\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Apical transport kinetics and transporter knockdown","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Citrulline uptake on permeable supports; SLC6A19 or SLC7A9 silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Renal evidence must not be relabeled as a demonstrated human intestinal transport mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Citrulline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HK-2 cells; rat cultures studied separately","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A neutral-amino-acid transporter contributes to kidney uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[citrulline-p19322909] Transport characteristics of L-citrulline in renal apical membrane of proximal tubular cells. 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