{"id":"78f1f380-866b-5cb4-9ed1-6cd7bfc828cd","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-oat1","predicate":"transports","statement":"Steviol trans-stimulated labeled para-aminohippurate efflux in human OAT1-expressing Xenopus oocytes, supporting transporter-mediated steviol movement.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ee3a8c86-f1d4-5c23-b371-6999bd24021b","mechanism_event_label":"A renal transporter recognizes the aglycone.","subject":{"id":"4872be13-147b-53b0-bd51-c717906aa430","slug":"slc22a6","display_name":"Human organic anion transporter 1 / SLC22A6","entity_type_key":"protein"},"object":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ee3a8c86-f1d4-5c23-b371-6999bd24021b","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-oat1-event","event_type":"observed_relationship","label":"A renal transporter recognizes the aglycone.","description":"Steviol trans-stimulated labeled para-aminohippurate efflux in human OAT1-expressing Xenopus oocytes, supporting transporter-mediated steviol movement.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4872be13-147b-53b0-bd51-c717906aa430","slug":"slc22a6","display_name":"Human organic anion transporter 1 / SLC22A6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cloned transporter in frog oocytes; 1 micromolar steviol trans-stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A heterologous transport assay is not a clinical renal clearance measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A renal transporter recognizes the aglycone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8). · 2005 · https://pubmed.ncbi.nlm.nih.gov/15644426/ · DOI 10.1124/jpet.104.080366","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"Inferred from trans-stimulation of a tracer's efflux, which shows movement without fixing its direction.","entity":null},{"dimension":"transport_pool","value_text":"the cytosol across the basolateral membrane","comparator":null,"unit":null,"notes":"Inferred from trans-stimulation of a tracer's efflux, which shows movement without fixing its direction.","entity":null}],"evidence":[{"id":"514b3769-fccf-5a9b-bae6-612c80deac9b","evidence_kind":"source_excerpt","locator":"Lines 98-104","start_line":98,"end_line":104,"excerpt":"## stevia-oat1\nA renal transporter recognizes the aglycone.\nSteviol trans-stimulated labeled para-aminohippurate efflux in human OAT1-expressing Xenopus oocytes, supporting transporter-mediated steviol movement.\nModel: Human cloned transporter in frog oocytes; 1 micromolar steviol trans-stimulation.\nLimitations: A heterologous transport assay is not a clinical renal clearance measurement.\nEvidence access: Primary abstract\nTransport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8). · 2005 · https://pubmed.ncbi.nlm.nih.gov/15644426/ · DOI 10.1124/jpet.104.080366","model_system":"Human cloned transporter in frog oocytes; 1 micromolar steviol trans-stimulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a36a82c2f8a0a971ea43e9e742db65f45848d13ab77310c0dd6b5cd55e31fc16","revision_id":"ea0355af-a926-511a-a7f2-1750baa95a8d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}