{"id":"78bcbea2-03d8-57ad-bd46-ee2fd908f33d","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:cldn16-depletion-selectivity","predicate":"reduces","statement":"Claudin-16 RNAi reduced paracellular cation selectivity in isolated mouse thick ascending limbs.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"81afb6ca-c02e-595b-b8db-fecde1a19eff","mechanism_event_label":"The junction lost part of its preference for positively charged ions, changing the conditions that support mineral recovery.","subject":{"id":"f7b8e03e-63ac-5f82-ad8d-e811391a8e29","slug":"mouse-cldn16-rnai-genotype","display_name":"Claudin-16 RNAi mouse genotype","entity_type_key":"gene"},"object":{"id":"1e74dd9d-7de5-57ec-8b45-95fe14a1bcc0","slug":"tal-paracellular-cation-selectivity","display_name":"TAL paracellular cation selectivity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"81afb6ca-c02e-595b-b8db-fecde1a19eff","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:cldn16-depletion-selectivity-event","event_type":"biochemical_relationship","label":"The junction lost part of its preference for positively charged ions, changing the conditions that support mineral recovery.","description":"Claudin-16 RNAi reduced paracellular cation selectivity in isolated mouse thick ascending limbs.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b9e564ce-7834-5815-a22b-0aa555a7bfe4","slug":"cldn16","display_name":"Claudin-16","entity_type_key":"protein"},"role":"depleted-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"permeability-probe","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"physiological-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"physiological-ion","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f7b8e03e-63ac-5f82-ad8d-e811391a8e29","slug":"mouse-cldn16-rnai-genotype","display_name":"Claudin-16 RNAi mouse genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"1e74dd9d-7de5-57ec-8b45-95fe14a1bcc0","slug":"tal-paracellular-cation-selectivity","display_name":"TAL paracellular cation selectivity","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"TAL electrical selectivity participates in magnesium and calcium conservation; no dietary synergy is implied.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence-system","value_text":"Renal RNAi and isolated TAL electrophysiology","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Renal RNAi and isolated TAL electrophysiology","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selectivity evidence is not proof claudin-16 alone forms a specifically magnesium-selective pore.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The junction lost part of its preference for positively charged ions, changing the conditions that support mineral recovery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hou-2007-cldn16] Transgenic RNAi depletion of claudin-16 and the renal handling of magnesium. (2007). https://pubmed.ncbi.nlm.nih.gov/17442678/ DOI: 10.1074/jbc.M700632200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Renal thick ascending limb","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal thick ascending limb","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3c3e3742-7245-514c-adca-b12314a4e2d2","evidence_kind":"source_excerpt","locator":"Lines 1097-1109","start_line":1097,"end_line":1109,"excerpt":"### cldn16-depletion-selectivity\nClaudin-16 RNAi reduced paracellular cation selectivity in isolated mouse thick ascending limbs.\nCondition category: machinery_impairment\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The junction lost part of its preference for positively charged ions, changing the conditions that support mineral recovery.\norganism: Mouse\ntissue_or_cell_type: Renal thick ascending limb\nexperimental_model: Renal RNAi and isolated TAL electrophysiology\nlimitations: Selectivity evidence is not proof claudin-16 alone forms a specifically magnesium-selective pore.\ncross_nutrient: TAL electrical selectivity participates in magnesium and calcium conservation; no dietary synergy is implied.\nevidence-system: Renal RNAi and isolated TAL electrophysiology\ntissue: Renal thick ascending limb\n[hou-2007-cldn16] Transgenic RNAi depletion of claudin-16 and the renal handling of magnesium. (2007). https://pubmed.ncbi.nlm.nih.gov/17442678/ DOI: 10.1074/jbc.M700632200","model_system":"Renal RNAi and isolated TAL electrophysiology","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hou-2007-cldn16] Transgenic RNAi depletion of claudin-16 and the renal handling of magnesium. (2007). https://pubmed.ncbi.nlm.nih.gov/17442678/ DOI: 10.1074/jbc.M700632200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}