{"id":"786d3d1b-7bbc-5f68-b6af-f2f436f8d862","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-lymphocyte-thiol-subset","predicate":"restores_low_thiol_subset_in","statement":"In peripheral blood lymphocytes, lipoic-acid treatment mainly restored a low-thiol subpopulation rather than raising all cells above physiological thiol levels.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"24bf9120-28fe-51fb-80be-63cc4a7a90f6","mechanism_event_label":"The response depended on the cells’ starting condition.","subject":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"object":{"id":"5e8dd2eb-2ad7-519b-a0a1-0ff9ffa705da","slug":"cellular-glutathione-pool","display_name":"Cellular reduced-glutathione pool","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"24bf9120-28fe-51fb-80be-63cc4a7a90f6","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-lymphocyte-thiol-subset-event","event_type":"observed_intervention","label":"The response depended on the cells’ starting condition.","description":"In peripheral blood lymphocytes, lipoic-acid treatment mainly restored a low-thiol subpopulation rather than raising all cells above physiological thiol levels.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5e8dd2eb-2ad7-519b-a0a1-0ff9ffa705da","slug":"cellular-glutathione-pool","display_name":"Cellular reduced-glutathione pool","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/9288403.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6a14c857d89c580ffe8866d7e5f06658dcc806429ae3a4b8ad4bd096f664a2e2\", \"start_char\": 0, \"end_char\": 1497, \"text_sha256\": \"6a14c857d89c580ffe8866d7e5f06658dcc806429ae3a4b8ad4bd096f664a2e2\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-culture thiol transport and glutathione experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lipoic acid/DHLA treatment and extracellular thiol measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-type and extracellular-substrate dependence; no demonstration of general methylation-vitamin depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human, rat and mouse cells as specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The response depended on the cells’ starting condition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p9288403] Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization. (1997). https://pubmed.ncbi.nlm.nih.gov/9288403/ DOI: 10.1002/biof.5520060303","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human lymphocytes, Jurkat cells and erythrocytes; rat C6 and mouse NB41A3 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9ccb707c-6bb1-5b74-a54c-467b13948bf2","evidence_kind":"source_excerpt","locator":"Lines 819-830","start_line":819,"end_line":830,"excerpt":"### ala-lymphocyte-thiol-subset\nIn peripheral blood lymphocytes, lipoic-acid treatment mainly restored a low-thiol subpopulation rather than raising all cells above physiological thiol levels.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The response depended on the cells’ starting condition.\norganism: Human, rat and mouse cells as specified\ntissue_or_cell_type: Human lymphocytes, Jurkat cells and erythrocytes; rat C6 and mouse NB41A3 cells\nexperimental_model: Cell-culture thiol transport and glutathione experiments\nlimitations: Cell-type and extracellular-substrate dependence; no demonstration of general methylation-vitamin depletion.\nexposure: Lipoic acid/DHLA treatment and extracellular thiol measurements\nevidence_span: {\"source_cache\": \"artifacts/ala-research/9288403.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6a14c857d89c580ffe8866d7e5f06658dcc806429ae3a4b8ad4bd096f664a2e2\", \"start_char\": 0, \"end_char\": 1497, \"text_sha256\": \"6a14c857d89c580ffe8866d7e5f06658dcc806429ae3a4b8ad4bd096f664a2e2\"}\n[ala-p9288403] Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization. (1997). https://pubmed.ncbi.nlm.nih.gov/9288403/ DOI: 10.1002/biof.5520060303","model_system":"Cell-culture thiol transport and glutathione experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ala-p9288403] Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization. (1997). https://pubmed.ncbi.nlm.nih.gov/9288403/ DOI: 10.1002/biof.5520060303","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d8afa8c2-ced9-5b28-90ca-2ac120ec7202","stable_key":"import-5d8e27d8-6a74-5560-827f-3f90908bbc34","title":"Alpha-lipoic acid: cofactor assembly, redox signaling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"611da198ab85a272eb26b64ee330ef6d1a762853a8481f3191c4acfca9f3cf3d","revision_id":"618b4ce6-3157-5222-8325-408064be5ea5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}