{"id":"7864b801-590d-5b6b-87cf-598cad757073","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-atf4-abundance","predicate":"increases_expression_of","statement":"Boric acid increased ATF4 protein at one hour in DU-145 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5291b215-6fb8-57ae-ad77-aaaa342a18ac","mechanism_event_label":"A regulator of the cellular stress response increased.","subject":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"object":{"id":"1a9dd2b3-2552-5387-a086-66bea9f56058","slug":"atf4","display_name":"Human activating transcription factor 4 / ATF4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5291b215-6fb8-57ae-ad77-aaaa342a18ac","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-atf4-abundance-event","event_type":"biochemical_relationship","label":"A regulator of the cellular stress response increased.","description":"Boric acid increased ATF4 protein at one hour in DU-145 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1a9dd2b3-2552-5387-a086-66bea9f56058","slug":"atf4","display_name":"Human activating transcription factor 4 / ATF4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/27587023.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\", \"start_char\": 0, \"end_char\": 1886, \"text_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Time-course immunoblotting and ER-stress gene expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 µM boric acid; early time points from 30 minutes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression changes do not establish direct molecular targets or clinical effects. Total eIF2α abundance is distinct from Ser51 phosphorylation. The study did not activate every ER-stress branch.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human DU-145 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A regulator of the cellular stress response increased.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p27587023] Activation of the EIF2α/ATF4 and ATF6 Pathways in DU-145 Cells by Boric Acid at the Concentration Reported in Men at the US Mean Boron Intake. (2017). https://pubmed.ncbi.nlm.nih.gov/27587023/ DOI: 10.1007/s12011-016-0824-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Prostate cancer cell culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8173e2ae-0542-5316-90b7-ec216e02d644","evidence_kind":"source_excerpt","locator":"Lines 391-402","start_line":391,"end_line":402,"excerpt":"### boron-atf4-abundance\nBoric acid increased ATF4 protein at one hour in DU-145 cells.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A regulator of the cellular stress response increased.\norganism: Human DU-145 cells\ntissue_or_cell_type: Prostate cancer cell culture\nexperimental_model: Time-course immunoblotting and ER-stress gene expression\nlimitations: Expression changes do not establish direct molecular targets or clinical effects. Total eIF2α abundance is distinct from Ser51 phosphorylation. The study did not activate every ER-stress branch.\nexposure: 10 µM boric acid; early time points from 30 minutes\nevidence_span: {\"source_cache\": \"artifacts/boron-research/27587023.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\", \"start_char\": 0, \"end_char\": 1886, \"text_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\"}\n[boron-p27587023] Activation of the EIF2α/ATF4 and ATF6 Pathways in DU-145 Cells by Boric Acid at the Concentration Reported in Men at the US Mean Boron Intake. 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