{"id":"78635ba7-4315-5ae6-b4e2-cce37ad16b33","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:acetylcoa-omission","predicate":"is_required_for_reported_assay","statement":"Omitting acetyl-CoA prevented the Dlat-associated HADHA acetylation signal in the purified-protein assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"43795690-323c-55cd-9c1e-9a2e591fd619","mechanism_event_label":"acetylcoa omission","subject":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"object":{"id":"f47c958c-2a43-5abc-8842-31700b1efa4c","slug":"hadha-construct-lysine-acetylation","display_name":"Acetyl-lysine signal on experimental HADHA constructs","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"43795690-323c-55cd-9c1e-9a2e591fd619","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:acetylcoa-omission","event_type":"is_required_for_reported_assay","label":"acetylcoa omission","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f47c958c-2a43-5abc-8842-31700b1efa4c","slug":"hadha-construct-lysine-acetylation","display_name":"Acetyl-lysine signal on experimental HADHA constructs","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80e2b56a-67d4-54a2-a847-2f55159ce64d","slug":"mouse-dlat","display_name":"Mouse dihydrolipoyl acetyltransferase / Dlat","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"652c711a-5098-5efe-8ccc-eb5cbd04b5e5","slug":"hadha-assay-construct","display_name":"HADHA experimental construct, species unresolved","entity_type_key":"protein"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"In vitro reaction, not an intact-cell compartment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Acetyl-CoA present/absent controls; concentrations and incubation details incompletely specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in the reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"hadha-construct-lysine-acetylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Acetyl-CoA omission control; Figure 6J","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant-protein acetylation assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"acetyl-coa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. This is an omission-control observation, not a dietary or intracellular acetyl-CoA threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Recombinant mouse Dlat; HADHA construct sequence species unresolved in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Omitting acetyl-CoA prevented the Dlat-associated HADHA acetylation signal in the purified-protein assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified-protein reaction","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e4b13f86-6480-567d-a224-99a633ae8d1e","evidence_kind":"source_excerpt","locator":"Lines 70-85","start_line":70,"end_line":85,"excerpt":"Omitting acetyl-CoA prevented the Dlat-associated HADHA acetylation signal in the purified-protein assay.\norganism: Recombinant mouse Dlat; HADHA construct sequence species unresolved in reviewed methods\ntissue_or_cell_type: Purified-protein reaction\nexperimental_model: Recombinant-protein acetylation assay\ncompartment: In vitro reaction, not an intact-cell compartment\ndose: Acetyl-CoA present/absent controls; concentrations and incubation details incompletely specified\nduration: Not specified in the reviewed methods\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Acetyl-CoA omission control; Figure 6J\nendpoint: hadha-construct-lysine-acetylation\nexposure: acetyl-coa\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. This is an omission-control observation, not a dietary or intracellular acetyl-CoA threshold.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]\nplain_language: Omitting acetyl-CoA prevented the Dlat-associated HADHA acetylation signal in the purified-protein assay.\nsample_size: 4 biological replicates","model_system":"Recombinant-protein acetylation assay","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}