{"id":"785494eb-3d57-59c2-9286-3665545152d5","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars2-rag","predicate":"promotes","statement":"TARS2 interacted with inactive Rag complexes, especially GTP-bound RagC, and promoted RagA GTP loading.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"35139a24-7d45-5e79-8d40-968a390e4b1c","mechanism_event_label":"A threonine-processing protein also links nutrient availability to a growth-control switch.","subject":{"id":"e1304998-8f29-56cc-8535-e65d11c53582","slug":"tars2","display_name":"Human mitochondrial threonyl-tRNA synthetase / TARS2","entity_type_key":"protein"},"object":{"id":"b0d6d634-a1ce-5015-a493-3276513e4ec0","slug":"human-raga-gtp-loading","display_name":"Human RagA GTP loading","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"35139a24-7d45-5e79-8d40-968a390e4b1c","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars2-rag-event","event_type":"observed_relationship","label":"A threonine-processing protein also links nutrient availability to a growth-control switch.","description":"TARS2 interacted with inactive Rag complexes, especially GTP-bound RagC, and promoted RagA GTP loading.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e1304998-8f29-56cc-8535-e65d11c53582","slug":"tars2","display_name":"Human mitochondrial threonyl-tRNA synthetase / TARS2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b0d6d634-a1ce-5015-a493-3276513e4ec0","slug":"human-raga-gtp-loading","display_name":"Human RagA GTP loading","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f025af3-f039-517f-908b-4af6fc65415e","slug":"mtorc1","display_name":"Mechanistic target of rapamycin complex 1","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7f8b948f-b375-5dd9-9ea9-1ba16d9c4f33","slug":"rragc","display_name":"Human RagC GTPase / RRAGC","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"7dee9af3-ddd2-58d7-ad78-4d7aed5e1ad7","slug":"rraga","display_name":"Human RagA GTPase / RRAGA","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cellular interaction and mTORC1 signaling experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"GTP-bound RagC here belongs to the inactive Rag configuration; GTP status has different implications for RagA and RagC.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A threonine-processing protein also links nutrient availability to a growth-control switch.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33340489/ · DOI 10.1016/j.molcel.2020.11.036","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"86e62abd-7615-5004-9ef0-9868e3c90284","evidence_kind":"source_excerpt","locator":"Lines 98-104","start_line":98,"end_line":104,"excerpt":"## l-threonine-tars2-rag\nA threonine-processing protein also links nutrient availability to a growth-control switch.\nTARS2 interacted with inactive Rag complexes, especially GTP-bound RagC, and promoted RagA GTP loading.\nModel: Cellular interaction and mTORC1 signaling experiments.\nLimitations: GTP-bound RagC here belongs to the inactive Rag configuration; GTP status has different implications for RagA and RagC.\nEvidence access: Primary abstract\nMitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33340489/ · DOI 10.1016/j.molcel.2020.11.036","model_system":"Cellular interaction and mTORC1 signaling experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3365aed939d9f567bd098449c7d67c7c00fb6c9ba163dcf34dc7cf6081f324fb","revision_id":"10a7b648-8dd7-5d4e-b618-c560a6e2d3c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}