{"id":"78171bd4-d0a6-5c0f-82d6-ac8467d24a4c","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-brain-pantothenate","predicate":"mediates_uptake","statement":"SLC5A6 knockdown analysis attributed 98.6% of total radiolabeled pantothenate uptake to SLC5A6 in human hCMEC/D3 brain endothelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e3009b7e-112f-53b1-b29c-9f76a8287fb3","mechanism_event_label":"SMVT supplied most measured pantothenate uptake in the human brain endothelial cell model.","subject":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"object":{"id":"ecc9f530-2005-5ced-9416-55e712928a8e","slug":"human-brain-endothelial-pantothenate-uptake","display_name":"Human brain endothelial pantothenate uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e3009b7e-112f-53b1-b29c-9f76a8287fb3","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-brain-pantothenate-event","event_type":"biochemical_relationship","label":"SMVT supplied most measured pantothenate uptake in the human brain endothelial cell model.","description":"SLC5A6 knockdown analysis attributed 98.6% of total radiolabeled pantothenate uptake to SLC5A6 in human hCMEC/D3 brain endothelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"cotransport_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ecc9f530-2005-5ced-9416-55e712928a8e","slug":"human-brain-endothelial-pantothenate-uptake","display_name":"Human brain endothelial pantothenate uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human and monkey brain microvessel analyses; SLC5A6-siRNA experiments in human hCMEC/D3 endothelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SLC5A6-specific siRNA and radiolabeled substrate uptake; tracer concentration not specified in the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cellular luminal uptake is not itself a measurement of complete transendothelial delivery into brain tissue. Reported percentages apply to hCMEC/D3 under these assay conditions, not every human tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SMVT supplied most measured pantothenate uptake in the human brain endothelial cell model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-brain2015] Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. (2015). https://pubmed.ncbi.nlm.nih.gov/25809983/ DOI: 10.1111/jnc.13092","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"hCMEC/D3 cerebral microvascular endothelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"770cbafc-8fc3-5d8a-82dc-24411c78c1ab","evidence_kind":"source_excerpt","locator":"Lines 275-286","start_line":275,"end_line":286,"excerpt":"### b5-trans-brain-pantothenate\nSLC5A6 knockdown analysis attributed 98.6% of total radiolabeled pantothenate uptake to SLC5A6 in human hCMEC/D3 brain endothelial cells.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: SMVT supplied most measured pantothenate uptake in the human brain endothelial cell model.\norganism: Homo sapiens\ntissue_or_cell_type: hCMEC/D3 cerebral microvascular endothelial cells\nexperimental_model: Human and monkey brain microvessel analyses; SLC5A6-siRNA experiments in human hCMEC/D3 endothelial cells\nlimitations: Cellular luminal uptake is not itself a measurement of complete transendothelial delivery into brain tissue. Reported percentages apply to hCMEC/D3 under these assay conditions, not every human tissue.\nexposure: SLC5A6-specific siRNA and radiolabeled substrate uptake; tracer concentration not specified in the abstract.\ncross_nutrient: true\n[b5-trans-brain2015] Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. (2015). https://pubmed.ncbi.nlm.nih.gov/25809983/ DOI: 10.1111/jnc.13092","model_system":"Human and monkey brain microvessel analyses; SLC5A6-siRNA experiments in human hCMEC/D3 endothelial cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-trans-brain2015] Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. (2015). https://pubmed.ncbi.nlm.nih.gov/25809983/ DOI: 10.1111/jnc.13092","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}