{"id":"7811c0f6-f858-544f-8c3f-2b61c70797a6","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-pagln-platelets","predicate":"enhances","statement":"PAGln at 100 micromolar for 30 minutes enhanced agonist-induced human platelet responses, including calcium signaling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b839451e-65be-5a53-9dc4-5b42ab11a6ae","mechanism_event_label":"A downstream microbial-host metabolite can alter platelet responsiveness in an assay.","subject":{"id":"8968a92b-40e9-56fe-8d76-d8776750dbd6","slug":"phenylacetylglutamine","display_name":"Phenylacetylglutamine / PAGln","entity_type_key":"small_molecule"},"object":{"id":"e7ef688b-c2fb-5ccd-8e05-dba12331cc50","slug":"human-pagln-platelet-responsiveness","display_name":"Human platelet agonist responsiveness during PAGln exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b839451e-65be-5a53-9dc4-5b42ab11a6ae","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-pagln-platelets-event","event_type":"observed_relationship","label":"A downstream microbial-host metabolite can alter platelet responsiveness in an assay.","description":"PAGln at 100 micromolar for 30 minutes enhanced agonist-induced human platelet responses, including calcium signaling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8968a92b-40e9-56fe-8d76-d8776750dbd6","slug":"phenylacetylglutamine","display_name":"Phenylacetylglutamine / PAGln","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e7ef688b-c2fb-5ccd-8e05-dba12331cc50","slug":"human-pagln-platelet-responsiveness","display_name":"Human platelet agonist responsiveness during PAGln exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human platelets; agonist-challenge experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Metabolite exposure is not phenylalanine exposure; this does not establish that dietary phenylalanine causes thrombosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A downstream microbial-host metabolite can alter platelet responsiveness in an assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8440bde5-d4b4-56a3-a64b-f3f67044b027","evidence_kind":"source_excerpt","locator":"Lines 374-380","start_line":374,"end_line":380,"excerpt":"## l-phenylalanine-pagln-platelets\nA downstream microbial-host metabolite can alter platelet responsiveness in an assay.\nPAGln at 100 micromolar for 30 minutes enhanced agonist-induced human platelet responses, including calcium signaling.\nModel: Isolated human platelets; agonist-challenge experiments.\nLimitations: Metabolite exposure is not phenylalanine exposure; this does not establish that dietary phenylalanine causes thrombosis.\nEvidence access: Primary full text\nA Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","model_system":"Isolated human platelets; agonist-challenge experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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